N7-guanine as a C+ mimic in hairpin aeg/aepPNA-DNA triplex:: Probing binding selectivity by UV-Tm and kinetics by fluorescence-based strand-invasion assay

被引：23

作者：

D'Costa, M ^{[1
]}

Kumar, VA ^{[1
]}

Ganesh, KN ^{[1
]}

机构：

[1] Natl Chem Lab, Div Organ Chem Synth, Pune 411008, Maharashtra, India

来源：

JOURNAL OF ORGANIC CHEMISTRY | 2003年 / 68卷 / 11期

关键词：

D O I：

10.1021/jo034048h

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

N7-substituted guanine (N7G) has been introduced into aminoethylglycyl bisPNA (7) as a C+ mimic to achieve pH-independent triplex formation with complementary DNA sequences. The introduction of chiral, cationic aminoethylprolyl units with C+ and C+ mimic N7G in the backbone of bisPNAs (8, 9) influenced the recognition of complementary DNA in an orientation-selective manner. A simple fluorescence assay is developed to examine the process of strand invasion of target DNA duplex by these modified bisPNAs and comparative results of the study employing triplex forming polypyrimidine (C/T) (6, 8) and purine-pyrimidine (N7G/T) mixmer-bisPNAs (7, 9) are presented.

引用

页码：4439 / 4445

页数：7