High-level tetracycline-resistant Neisseria gonorrhoeae in Ontario, Canada - Investigation of a cluster of isolates, showing chromosomally mediated resistance to penicillin combined with plasmid-mediated resistance to tetracycline

被引:7
作者
Harnett, N
Brown, S
Terro, R
Krishnan, C
Pauze, R
Yeung, KH
机构
[1] ONTARIO MINIST HLTH,CENT PUBL HLTH LAB,TORONTO,ON M5W 1R5,CANADA
[2] HLTH CANADA,NATL LAB SEXUALLY TRANSMITTED DIS,LAB CTR DIS CONTROL,OTTAWA,ON K1A 0L2,CANADA
关键词
D O I
10.1086/514122
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Between 1991 and 1994, plasmid-mediated, tetracycline-resistant Neisseria gonorrhoeae (TRNG) increased from 61.8% to 85.96% of all resistant isolates in Ontario, Canada, Ninety-nine isolates with tetracycline MICs > 32 mg/L were characterized by auxotype/serovar (A/S) class, plasmid profile, hybridization with eight tetracycline-resistant probes, and pulsed-field gel electrophoresis (PFGE) of genomic DNA after digestion with NheI and SpeI restriction endonucleases. A cluster of 82 isolates with penicillin MICs of 2-4 mg/L and tetracycline MICs of 128 mg/L (chromosomally mediated resistance) belonged to A/S class NR/IB-1 and had identical or closely related PFGE profiles, Seventeen isolates, TRNG (10) and penicillinase-producing TRNG (7), with tetracycline MICs of 64-256 mg/L, belonged to eight A/S classes and displayed 12 different PFGE profiles, The 99 isolates hybridized only with the TetM probe, Phenotypic and molecular characterization indicated a diverse population throughout the Province of Ontario.
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页码:1269 / 1276
页数:8
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