Silencing of unpaired chromatin and histone H2A ubiquitination in mammalian meiosis

被引：237

作者：

Baarends, WM

Wassenaar, E

van der Laan, R

Hoogerbrugge, J

Sleddens-Linkels, E

Hoeijmakers, JHJ

de Boer, P

Grootegoed, JA

机构：

[1] Erasmus Univ, Med Ctr, Dept Reprod & Dev, NL-3000 DR Rotterdam, Netherlands

[2] Erasmus Univ, Med Ctr, MGC Dept Cell Biol & Genet, NL-3000 DR Rotterdam, Netherlands

[3] Univ St Radboud, Med Ctr, Dept Obstet & Gynaecol, Nijmegen, Netherlands

来源：

MOLECULAR AND CELLULAR BIOLOGY | 2005年 / 25卷 / 03期

关键词：

D O I：

10.1128/MCB.25.3.1041-1053.2005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During meiotic prophase in male mammals, the X and Y chromosomes are incorporated in the XY body. This heterochromatic body is transcriptionally silenced and marked by increased ubiquitination of histone H2A. This led us to investigate the relationship between histone H2A ubiquitination and chromatin silencing in more detail. First, we found that ubiquitinated H2A also marks the silenced X chromosome of the Barr body in female somatic cells. Next, we studied a possible relationship between H2A ubiquitination. chromatin silencing. and unpaired chromatin in meiotic prophase. The mouse models used carry an unpaired autosomal region in male meiosis or unpaired X and Y chromosomes in female meiosis. We show that ubiquitinated histone H2A is associated with transcriptional silencing of large chromatin regions. This silencing in mammalian meiotic prophase cells concerns unpaired chromatin regions and resembles a phenomenon described for the fungus Neurospora crassa and named meiotic silencing by unpaired DNA.

引用

页码：1041 / 1053

页数：13

共 56 条

[11] MALE PACHYTENE PAIRING IN SINGLE AND DOUBLE TRANSLOCATION HETEROZYGOTES AND SPERMATOGENIC IMPAIRMENT IN THE MOUSE
DEBOER, P
SEARLE, AG
VANDERHOEVEN, FA
DEROOIJ, DG
BEECHEY, CV
[J]. CHROMOSOMA, 1986, 93 (04) : 326 - 336
[12] Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6
Dover, J
Schneider, J
Tawiah-Boateng, MA
Wood, A
Dean, K
Johnston, M
Shilatifard, A
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (32) : 28368 - 28371
[13] H2AX is required for chromatin remodeling and inactivation of sex chromosomes in male mouse meiosis
Fernandez-Capetillo, O
Mahadevaiah, SK
Celeste, A
Romanienko, PJ
Camerini-Otero, RD
Bonner, WM
Manova, K
Burgoyne, P
Nussenzweig, A
[J]. DEVELOPMENTAL CELL, 2003, 4 (04) : 497 - 508
[14] 3-DIMENSIONAL RECONSTRUCTION OF HUMAN PACHYTENE SPERMATOCYTE NUCLEI OF A 17-21 RECIPROCAL TRANSLOCATION CARRIER - STUDY OF XY-AUTOSOME RELATIONSHIPS
GUICHAOUA, MR
DELANVERSIN, A
CATALDO, C
DELAFONTAINE, D
ALASIA, C
FRATERNO, M
TERRIOU, P
STAHL, A
LUCIANI, JM
[J]. HUMAN GENETICS, 1991, 87 (06) : 709 - 715
[15] Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8
Henry, KW
Wyce, A
Lo, WS
Duggan, LJ
Emre, NCT
Kao, CF
Pillus, L
Shilatifard, A
Osley, MA
Berger, SL
[J]. GENES & DEVELOPMENT, 2003, 17 (21) : 2648 - 2663
[16] Synaptonemal complexes: Structure and function
Heyting, C
[J]. CURRENT OPINION IN CELL BIOLOGY, 1996, 8 (03) : 389 - 396
[17] Histone MacroH2A1.2 is concentrated in the XY-body by the early pachytene stage of spermatogenesis
Hoyer-Fender, S
Costanzi, C
Pehrson, JR
[J]. EXPERIMENTAL CELL RESEARCH, 2000, 258 (02) : 254 - 260
[18] Inheritance of a pre-inactivated paternal X chromosome in early mouse embryos
Huynh, KD
Lee, JT
[J]. NATURE, 2003, 426 (6968) : 857 - 862
[19] Detection of unpaired DNA at meiosis results in RNA-mediated silencing
Hynes, MJ
Todd, RB
[J]. BIOESSAYS, 2003, 25 (02) : 99 - 103
[20] Kelly WG, 1998, DEVELOPMENT, V125, P2451

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