On-line HPLC-ABTS screening and HPLC-DAD-MS/MS identification of free radical scavengers in Gardenia (Gardenia jasminoides Ellis) fruit extracts

被引：135

作者：

He, Wenhao ^{[1
]}

Liu, Xuan ^{[1
]}

Xu, Honggao ^{[1
]}

Gong, Ying ^{[1
]}

Yuan, Fang ^{[1
]}

Gao, Yanxiang ^{[1
]}

机构：

[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

来源：

FOOD CHEMISTRY | 2010年 / 123卷 / 02期

关键词：

Gardenia fruit extracts; Phenolic compounds; Free radical scavenging activity; On-line HPLC-ABTS; HPLC-DAD-MS/MS; CROCUS-SATIVUS STIGMAS; ANTIOXIDANT ACTIVITY; PHENOLIC-COMPOUNDS; ACID-DERIVATIVES; LIQUID-CHROMATOGRAPHY; CHLOROGENIC ACIDS; LC-MSN; CONSTITUENTS; ISOMERS; FRUCTUS;

D O I：

10.1016/j.foodchem.2010.04.030

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Gardenia jasminoides Ellis is a folk medicine for pharmacological activities in China. In this study, on-line HPLC-ABTS and HPLC-DAD-MS/MS analyses were applied to screen and identify free radical scavengers in gardenia fruit extracts (GFE). Nine compounds were identified (3-caffeoylquinic acid, 4-caffeoylquinic acid, 5-caffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, quercetin-3-rutinoside, crocetin di(beta-gentiobiosyl) ester, and geniposide). 3-Caffeoylquinic acid and 4-caffeoylquinic acid were firstly found in GFE. Caffeoylquinic acids, dicaffeoylquinic acids, and 4-sinapoyl-5-caffeoylquinic acid were the dominant free radical scavengers in GFE, and their Trolox equivalent antioxidant capacities (TEAC) were determined. 3,5-Dicaffeoylquinic acid was the most potent antioxidant with TEAC value of 1.1 mM. In contrast, crocetin di( p-gentiobiosyl) ester and geniposide showed little ABTS.(+) scavenging activity. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：521 / 528

页数：8

共 31 条

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