Expression, purification and crystallization of human prolylcarboxypeptidase

被引：15

作者：

Abeywickrema, Pravien D. ^{[1
]}

Patel, Sangita B. ^{[1
]}

Byrne, Noel J. ^{[1
]}

Diehl, Ronald E. ^{[1
]}

Hall, Dawn L. ^{[1
]}

Ford, Rachael E. ^{[1
]}

Rickert, Keith W. ^{[1
]}

Reid, John C. ^{[1
]}

Shipman, Jennifer M. ^{[1
]}

Geissler, Wayne M. ^{[2
]}

Pryor, Kelly D. ^{[2
]}

SinhaRoy, Ranabir ^{[2
]}

Soisson, Stephen M. ^{[1
]}

Lumb, Kevin J. ^{[1
]}

Sharma, Sujata ^{[1
]}

机构：

[1] Merck Res Labs, Global Struct Biol, West Point, PA 19486 USA

[2] Merck Res Labs, Dept Metab Disorders, Rahway, NJ 07065 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2010年 / 66卷

关键词：

ANGIOTENSINASE-C; PREKALLIKREIN;

D O I：

10.1107/S1744309110014041

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Prolylcarboxypeptidase (PrCP) is a lysosomal serine carboxypeptidase that cleaves a variety of C-terminal amino acids adjacent to proline and has been implicated in diseases such as hypertension and obesity. Here, the robust production, purification and crystallization of glycosylated human PrCP from stably transformed CHO cells is described. Purified PrCP yielded crystals belonging to space group R32, with unit-cell parameters a = b = 181.14, c = 240.13 angstrom, that diffracted to better than 2.8 angstrom resolution.

引用

页码：702 / 705

页数：4

共 9 条

[1] Prolylcarboxypeptidase: A cardioprotective enzyme [J].

Mallela, J. ;

Yang, J. ;

Shariat-Madar, Z. .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2009, 41 (03) :477-481

[2] SOLVENT CONTENT OF PROTEIN CRYSTALS [J].

MATTHEWS, BW .

JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) :491-+

[3]

ODYA CE, 1978, J BIOL CHEM, V253, P5927

[4] Processing of X-ray diffraction data collected in oscillation mode [J].

Otwinowski, Z ;

Minor, W .

MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 :307-326

[5] Recombinant prolylcarboxypeptidase activates plasma prekallikrein [J].

Shariat-Madar, Z ;

Mahdi, F ;

Schmaier, AH .

BLOOD, 2004, 103 (12) :4554-4561

[6] Identification and characterization of prolylcarboxypeptidase as an endothelial cell prekallikrein activator [J].

Shariat-Madar, Z ;

Mahdi, F ;

Schmaier, AH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (20) :17962-17969

[7] Structural Basis of Human p70 Ribosomal S6 Kinase-1 Regulation by Activation Loop Phosphorylation [J].

Sunami, Tomoko ;

Byrne, Noel ;

Diehl, Ronald E. ;

Funabashi, Kaoru ;

Hall, Dawn L. ;

Ikuta, Mari ;

Patel, Sangita B. ;

Shipman, Jennifer M. ;

Smith, Robert F. ;

Takahashi, Ikuko ;

Zugay-Murphy, Joan ;

Iwasawa, Yoshikazu ;

Lumb, Kevin J. ;

Munshi, Sanjeev K. ;

Sharma, Sujata .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (07) :4587-4594

[8]

TAN FL, 1993, J BIOL CHEM, V268, P16631

[9] Prolylcarboxypeptidase regulates food intake by inactivating α-MSH in rodents [J].

Wallingford, Nicholas ;

Perroud, Bertrand ;

Gao, Qian ;

Coppola, Anna ;

Gyengesi, Erika ;

Liu, Zhong-Wu ;

Gao, Xiao-Bing ;

Diament, Adam ;

Haus, Kari A. ;

Shariat-Madar, Zia ;

Mahdi, Fakhri ;

Wardlaw, Sharon L. ;

Schmaier, Alvin H. ;

Warden, Craig H. ;

Diano, Sabrina .

JOURNAL OF CLINICAL INVESTIGATION, 2009, 119 (08) :2291-2303

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