PUNS:: transcriptomic- and genomic-in silico PCR for enhanced primer design

被引：18

作者：

Boutros, PC ^{[1
]}

Okey, AB ^{[1
]}

机构：

[1] Univ Toronto, Dept Pharmacol, Toronto, ON M5S 1A8, Canada

来源：

BIOINFORMATICS | 2004年 / 20卷 / 15期

基金：

加拿大健康研究院;

关键词：

D O I：

10.1093/bioinformatics/bth257

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a CGI/Perl-based web server to perform in silico polymerase chain reaction (PCR) on PCR primer sequences. The PUNS (Primer-UniGene Selectivity) server simulates PCR reactions by running BLASTN analysis on user-entered primer pairs against both the transcriptome and the genome to assess primer specificity. PUNS is particularly suited for the identification of highly selective primers for quantitative microarray validation.

引用

页码：2399 / 2400

页数：2

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