Distance Determination in Proteins inside Xenopus laevis Oocytes by Double Electron-Electron Resonance Experiments

被引：99

作者：

Igarashi, Ryuji ^{[1
,2
]}

Sakai, Tomomi ^{[3
]}

Hara, Hideyuki ^{[4
]}

Tenno, Takeshi ^{[2
,5
]}

Tanaka, Toshiaki ^{[6
]}

Tochio, Hidehito ^{[1
,2
]}

Shirakawa, Masahiro ^{[1
,2
,7
]}

机构：

[1] Kyoto Univ, Grad Sch Engn, Kyoto 6158510, Japan

[2] JST, CREST, Kawaguchi, Saitama 3320012, Japan

[3] Mitsubishi Kagaku Inst Life Sci, Tokyo 1948511, Japan

[4] Bruker Biospin KK, Kanagawa 2210022, Japan

[5] Kobe Univ, Grad Sch Med, Kobe, Hyogo 6500017, Japan

[6] Tokyo Inst Technol, Fac Biosci & Biotechnol, Kanagawa 2268501, Japan

[7] Yokohama Inst, RIKEN, Kanagawa 2300045, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 24期

基金：

日本科学技术振兴机构;

关键词：

CELL NMR-SPECTROSCOPY; ACTIVATION; ESR;

D O I：

10.1021/ja906104e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

DEER (double electron-electron resonance) enables the observation of long-range dipole interactions (1.5-8 nm) between electron spin centers and has become a unique method for structural analysis of site-directed spin-labeled (SDSL) proteins. The method was applied to proteins inside eukaryotic cells, Xenopus laevis oocytes. DEER measurements of the oocytes, into which SDSL-ubiquitin derivates were injected, gave rise to interpretable signals and allowed us to perform in situ analyses of the interspin distances of the proteins.

引用

页码：8228 / +

页数：4

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