Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR)

被引：126

作者：

Burz, DS

Dutta, K

Cowburn, D

Shekhtman, A

机构：

[1] SUNY Albany, Dept Chem, Albany, NY 12222 USA

[2] New York Struct Biol Ctr, New York, NY 10027 USA

来源：

NATURE METHODS | 2006年 / 3卷 / 02期

关键词：

D O I：

10.1038/NMETH851

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a high-throughput in-cell nuclear magnetic resonance (NMR)-based method for mapping the structural changes that accompany protein-protein interactions (STINT-NMR). The method entails sequentially expressing two (or more) proteins within a single bacterial cell in a time-controlled manner and monitoring the protein interactions using in-cell NMR spectroscopy. The resulting spectra provide a complete titration of the interaction and define structural details of the interacting surfaces at atomic resolution.

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收藏

页码：91 / 93

页数：3

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