Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp OU01

被引：38

作者：

Sun, Yuying

Han, Baoqin

Liu, Wanshun ^{[1
]}

Zhang, Jiquan

Gao, Xingshuang

机构：

[1] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

[2] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2007年 / 98卷 / 08期

基金：

国家高技术研究发展计划(863计划);

关键词：

chitosanase; induction; colloidal chitosan; statistical optimization; Plackett-Burman; response surface methodology;

D O I：

10.1016/j.biortech.2006.07.020

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by Microbacteritan sp. OU01 in shake-flask. A significant influence of (NH4)(2)SO4, MgSO4 center dot 7H(2)O and initial pH on chitosanase production was noted with Plackett-Burman design. It was then revealed with the method of steepest ascent and response surface methodology (RSM) that 19.0 g/L (NH4)(2)SO4, 1.3 g/L MgSO4 and an initial pH of 2.0 were optimum for the production of chitosanase; colloidal chitosan appeared to be the best inducer for chitosanase production by Microbacterium sp. OU01. This optimization strategy led to the enhancement of chitosanase from 3.6 U/mL to 118 U/mL. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：1548 / 1553

页数：6