Measuring gene expression by quantitative proteome analysis

被引：115

作者：

Gygi, SP ^{[1
]}

Rist, B ^{[1
]}

Aebersold, R ^{[1
]}

机构：

[1] Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2000年 / 11卷 / 04期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/S0958-1669(00)00116-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the precise quantification and identification of proteins in complex mixtures, that is, quantitative proteomics.

引用

页码：396 / 401

页数：6

共 28 条

[21]

OFARRELL PH, 1975, J BIOL CHEM, V250, P4007

[22] High throughput proteome-wide precision measurements of protein expression using mass spectrometry [J].

Pasa-Tolic, L ;

Jensen, PK ;

Anderson, GA ;

Lipton, MS ;

Peden, KK ;

Martinovic, S ;

Tolic, N ;

Bruce, JE ;

Smith, RD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (34) :7949-7950

[23] MASS-SPECTROMETRIC APPROACHES FOR THE IDENTIFICATION OF GEL-SEPARATED PROTEINS [J].

PATTERSON, SD ;

AEBERSOLD, R .

ELECTROPHORESIS, 1995, 16 (10) :1791-1814

[24]

Perrot M, 1999, ELECTROPHORESIS, V20, P2280, DOI 10.1002/(SICI)1522-2683(19990801)20:11<2280::AID-ELPS2280>3.3.CO

[25]

2-H

[26] A strategy for rapid, high confidence protein identification [J].

Qin, J ;

Fenyo, D ;

Zhao, YM ;

Hall, WW ;

Chao, DM ;

Wilson, CJ ;

Young, RA ;

Chait, BT .

ANALYTICAL CHEMISTRY, 1997, 69 (19) :3995-4001

[27]

Shabanowitz J, 2000, MASS SPECTROMETRY IN BIOLOGY & MEDICINE, P163

[28] Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gels [J].

Shevchenko, A ;

Jensen, ON ;

Podtelejnikov, AV ;

Sagliocco, F ;

Wilm, M ;

Vorm, O ;

Mortensen, P ;

Shevchenko, A ;

Boucherie, H ;

Mann, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14440-14445

← 1 2 3 →