Pectenotoxin-2 seco acid:: a toxin converted from pectenotoxin-2 by the New Zealand Greenshell mussel, Perna canaliculus

被引:91
作者
Suzuki, T
Mackenzie, L
Stirling, D
Adamson, J
机构
[1] Tohoku Natl Fisheries Res Inst, Shiogama, Miyagi 9850001, Japan
[2] Cawthron Inst, Nelson, New Zealand
[3] Inst Environm Sci & Res Ltd, Porirua, New Zealand
关键词
pectenotoxin-2; pectenotoxin-2 seco acid; Greenshell mussel; LC-MS; diarrheic shellfish poisoning toxin;
D O I
10.1016/S0041-0101(00)00153-7
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Comparison of pectenotoxin (PTX) profiles of toxic dinoflagellate Dinophysis acuta, Greenshell mussels (Perna canaliculus) and Blue mussels (Mytilus galloprovincialis) collected from Wedge Point, Queen Charlotte Sound, New Zealand was carried out by liquid chromatography-mass spectrometry with turbo-ionspray ionization. Although the major PTX homologue in D. acuta was pectenotoxin-2 (PTX2), both Greenshell and Blue mussels contained pectenotoxin-2 seco acid (PTX2SA) as the predominant toxin. More than 90% of PTX2 isolated from D. acuta was rapidly converted to PTX2SA and its epimer 7-epi-pectenotoxin-2 seco acid (7-epi-PTX2SA) in the Greenshell mussel extracts. The conversion from PTX2 to PTX2SA and 7-epi-PTX2SA was not observed in phosphate buffers at various pH ranging from 4.1 to 9.1. These findings indicate that PTX2SA and 7-epi-PTX2SA are not artifact toxins resulting from hydrolysis of PTX2, but arise from the conversion of PTX2 by mussel tissues. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:507 / 514
页数:8
相关论文
共 20 条
[1]   Structures of new pectenotoxin analogs, pectenotoxin-2 seco acid and 7-epi-pectenotoxin-2 seco acid, isolated from a dinoflagellate and greenshell mussels [J].
Daiguji, M ;
Satake, M ;
James, KJ ;
Bishop, A ;
MacKenzie, L ;
Naoki, H ;
Yasumoto, T .
CHEMISTRY LETTERS, 1998, (07) :653-654
[2]   New approach to the direct detection of known and new diarrhoeic shellfish toxins in mussels and phytoplankton by liquid chromatography-mass spectrometry [J].
Draisci, R ;
Palleschi, L ;
Giannetti, L ;
Lucentini, L ;
James, KJ ;
Bishop, AG ;
Satake, M ;
Yasumoto, T .
JOURNAL OF CHROMATOGRAPHY A, 1999, 847 (1-2) :213-221
[3]   First report of pectenotoxin-2 (PTX-2) in algae (Dinophysis fortii) related to seafood poisoning in Europe [J].
Draisci, R ;
Lucentini, L ;
Giannetti, L ;
Boria, P ;
Poletti, R .
TOXICON, 1996, 34 (08) :923-935
[4]  
GOTO H, 1998, HARMFUL ALGAE, P216
[5]   Liquid chromatographic methods for the isolation and identification of new pectenotoxin-2 analogues from marine phytoplankton and shellfish [J].
James, KJ ;
Bishop, AG ;
Draisci, R ;
Palleschi, L ;
Marchiafava, C ;
Ferretti, E ;
Satake, M ;
Yasumoto, T .
JOURNAL OF CHROMATOGRAPHY A, 1999, 844 (1-2) :53-65
[6]   Cytotoxic compounds from a two-sponge association [J].
Jung, JH ;
Sim, CJ ;
Lee, CO .
JOURNAL OF NATURAL PRODUCTS, 1995, 58 (11) :1722-1726
[7]   Determination of diarrhetic shellfish toxins in various dinoflagellate species [J].
Lee, Jong-Soo ;
Igarashi, Teruo ;
Fraga, Santiago ;
Dahl, Einal ;
Hovgaard, Peter ;
Yasumotol, Takeshi .
JOURNAL OF APPLIED PHYCOLOGY, 1989, 1 (02) :147-152
[8]   FLUOROMETRIC-DETERMINATION OF DIARRHETIC SHELLFISH TOXINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
LEE, JS ;
YANAGI, T ;
KENMA, R ;
YASUMOTO, T .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1987, 51 (03) :877-881
[9]  
LEE JS, 1989, BIOACT MOL, V10, P327
[10]  
MacKenzie L., 1998, Harmful Algae, P74