Formation of the spore photoproduct and other dimeric lesions between adjacent pyrimidines in UVC-irradiated dry DNA

被引：42

作者：

Douki, T ^{[1
]}

Cadet, J ^{[1
]}

机构：

[1] CEA Grenoble, Dept Rech Fondamentale Mat Condensee, DSM,FRE 2600, Serv Chim Inorgan & Biol,Lab Les Acides Nucleique, F-38054 Grenoble 9, France

来源：

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES | 2003年 / 2卷 / 04期

关键词：

D O I：

10.1039/b300173c

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Far-UV irradiation of DNA leads to the formation of several types of dimeric lesions between adjacent pyrimidine bases including cyclobutane dimers, (6-4) photoproducts and Dewar valence isomers. In the dry state, an additional specific thymine lesion, the spore photoproduct, is produced. We designed an HPLC-tandem mass spectrometry assay for the detection of the latter lesion. This technique that does not require radio-labelling of DNA allowed the simultaneous quantification of the spore photoproduct and other pyrimidine dimeric photoproducts. Using this approach, the complete distribution of bipyrimidine lesions within UVC-irradiated dry DNA was determined.

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页码：433 / 436

页数：4

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