The IGF-I splice variant MGF increases progenitor cells in ALS, dystrophic, and normal muscle

被引:80
作者
Ates, Kenan
Yang, Shi Yu
Orrell, Richard W.
Sinanan, Andrea C. M.
Simons, Paul
Solomon, Andrew
Beech, Steven
Goldspink, Geoffrey
Lewis, Mark P.
机构
[1] UCL, Div Biomat & Tissue Engn, Eastman Dent Inst, London WC1X 8LD, England
[2] UCL, Royal Free & Univ Coll Med Sch, Dept Anat & Dev Biol, London W1N 8AA, England
基金
英国惠康基金;
关键词
muscle stem; satellite cells; ALS; muscular dystrophy; insulin-like growth factor I; mechano growth factor;
D O I
10.1016/j.febslet.2007.05.030
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of muscle splice variants of insulin-like growth factor I (IGF-I) on proliferation and differentiation were studied in human primary muscle cell cultures from healthy subjects as well as from muscular dystrophy and ALS patients. Although the initial numbers of mononucleated progenitor cells expressing desmin were lower in diseased muscle, the E domain peptide of IGF-IEc (MGF) significantly increased the numbers of progenitor cells in healthy and diseased muscle. IGF-I significantly enhances myogenic differentiation whereas MGF E peptide blocks this pathway, resulting in an increased progenitor (stem) cell pool and thus potentially facilitating repair and maintenance of this postmitotic tissue. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:2727 / 2732
页数:6
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