Mining the active proteome in plant science and biotechnology

被引：27

作者：

Kolodziejek, Izabella ^{[1
,2
]}

van der Hoorn, Renier A. L. ^{[1
,2
]}

机构：

[1] Max Planck Inst Plant Breeding Res, Plant Chemet Lab, D-50829 Cologne, Germany

[2] Max Planck Gesell, Chem Genom Ctr, D-44227 Dortmund, Germany

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2010年 / 21卷 / 02期

关键词：

SERINE HYDROLASE ACTIVITIES; DISEASE RESISTANCE; CYSTEINE PROTEASES; ENZYME; TARGETS; IDENTIFICATION; COMPLEX; PROBES; TOOLS; VPE;

D O I：

10.1016/j.copbio.2010.02.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein activity is essential functional information, yet difficult to predict from transcript or protein data. Activity-based protein profiling (ABPP) displays active proteins in proteomes using small molecule probes that irreversibly label proteins in their active state. Here, we review proof-of-concept ABPP studies in plant science. These studies displayed activities of dozens of plant cysteine proteases, lipases, methylesterases and the proteasome. ABPP in plants revealed differential protein activities in development and immunity and uncovered striking selectivity of pathogen-derived inhibitors and unexpected targets of commercial inhibitors. The unique, high-content information of ABPP and the robustness and simplicity of the assays will make ABPP a powerful tool in future plant science and biotechnology.

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收藏

页码：225 / 233

页数：9

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