Differentiation of a porcine reproductive and respiratory syndrome virus vaccine strain from North American field strains by restriction fragment length polymorphism analysis of ORF 5

被引：131

作者：

Wesley, RD

Mengeling, WL

Lager, KM

Clouser, DF

Landgraf, JG

Frey, ML

机构：

[1] USDA ARS, Natl Anim Dis Ctr, Virol Swine Res Unit, Ames, IA 50010 USA

[2] USDA, Diagnot Virol Lab, Natl Vet Serv Labs, Anim & Plant Hlth Inspect Serv, Ames, IA 50010 USA

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1998年 / 10卷 / 02期

关键词：

D O I：

10.1177/104063879801000204

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from other North American field strains was investigated. Open reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 restriction enzyme gel patterns characterize the vaccine virus and the parent strain genotype. The combined 3 RFLP patterns differentiate the vaccine and parent virus from other PRRSV strains. This test will be a valuable tool in epidemiologic studies and will be useful in identifying individual strains in cases of multistrain PRRSV infections.

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页码：140 / 144

页数：5

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