Electrochemical detection of protein-protein interactions using a yeast two hybrid:: 17-β-estradiol as a model

被引：7

作者：

Schwartz-Mittelmann, A ^{[1
]}

Neufeld, T ^{[1
]}

Biran, D ^{[1
]}

Rishpon, J ^{[1
]}

机构：

[1] Tel Aviv Univ, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, Israel

来源：

ANALYTICAL BIOCHEMISTRY | 2003年 / 317卷 / 01期

关键词：

yeast two hybrid; estradiol; electrochemical; protein-protein interaction;

D O I：

10.1016/S0003-2697(03)00110-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work we present a modified yeast two-hybrid bioassay for the highly sensitive detection of protein-protein interactions, based on the electrochemical monitoring of beta-D-galactosidase reporter gene activity, using p-aminophenyl-beta-D-galactopyranoside (PAPG) as a synthetic substrate. In a model system, the sensitive detection of 17-beta-estradiol was achieved at concentrations as low as 10(-11) M (approx 2 pg/ml) by monitoring 17-beta-estradiol receptor dimerization after exposure to 17-beta-estradiol. The sensitivity of this system was higher than that of standard optical methods by three orders of magnitude. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：34 / 39

页数：6

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