The role of angiopoietins in the development of endothelial cells from cord blood CD34+ progenitors

被引:76
作者
Hildbrand, P
Cirulli, V
Prinsen, RC
Smith, KA
Torbett, BE
Salomon, DR
Crisa, L
机构
[1] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA
[2] Univ Calif San Diego, Dept Pediat, La Jolla, CA 92093 USA
关键词
D O I
10.1182/blood-2003-12-4219
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Circulating endothelial progenitors contribute to neovascularization at sites of injury and tumorigenesis in postnatal life. Yet, the molecular mechanisms initiating the endothelial developmental program of these precursors remain elusive. Here we provide evidence that endothelial development from progenitors circulating in human cord blood requires angiopoietins, a set of growth factors also involved in vascular branching during embryogenesis. We show that cord blood cells with the potential for endothelial development reside in a CD34(+)CD11b(+) subset capable of autonomously producing and binding angiopoietins. Functionally, endogenous angiopoietin-1 regulates initial endothelial cell commitment, whereas angiopoietin-2 enhances expansion of the endothelial cell progeny. These findings suggest a role for angiopoietins as regulators of endothelial development from circulating progenitors and imply a function of angiopoietins at distinct developmental steps in postnatal angiogenesis. (C) 2004 by The American Society of Hematology.
引用
收藏
页码:2010 / 2019
页数:10
相关论文
共 45 条
  • [1] Isolation of putative progenitor endothelial cells for angiogenesis
    Asahara, T
    Murohara, T
    Sullivan, A
    Silver, M
    vanderZee, R
    Li, T
    Witzenbichler, B
    Schatteman, G
    Isner, JM
    [J]. SCIENCE, 1997, 275 (5302) : 964 - 967
  • [2] Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization
    Asahara, T
    Masuda, H
    Takahashi, T
    Kalka, C
    Pastore, C
    Silver, M
    Kearne, M
    Magner, M
    Isner, JM
    [J]. CIRCULATION RESEARCH, 1999, 85 (03) : 221 - 228
  • [3] Expression of Tie1, Tie2, and angiopoietins 1, 2, and 4 in Kaposi's sarcoma and cutaneous angiosarcoma
    Brown, LF
    Dezube, BJ
    Tognazzi, K
    Dvorak, HF
    Yancopoulos, GD
    [J]. AMERICAN JOURNAL OF PATHOLOGY, 2000, 156 (06) : 2179 - 2183
  • [4] Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele
    Carmeliet, P
    Ferreira, V
    Breier, G
    Pollefeyt, S
    Kieckens, L
    Gertsenstein, M
    Fahrig, M
    Vandenhoeck, A
    Harpal, K
    Eberhardt, C
    Declercq, C
    Pawling, J
    Moons, L
    Collen, D
    Risau, W
    Nagy, A
    [J]. NATURE, 1996, 380 (6573) : 435 - 439
  • [5] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
  • [6] Human cord blood progenitors sustain thymic T-cell development and a novel form of angiogenesis
    Crisa, L
    Cirulli, V
    Smith, KA
    Ellisman, MH
    Torbett, BE
    Salomon, DR
    [J]. BLOOD, 1999, 94 (11) : 3928 - 3940
  • [7] Isolation of Angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning
    Davis, S
    Aldrich, TH
    Jones, PF
    Acheson, A
    Compton, DL
    Jain, V
    Ryan, TE
    Bruno, J
    Radziejewski, C
    Maisonpierre, PC
    Yancopoulos, GD
    [J]. CELL, 1996, 87 (07) : 1161 - 1169
  • [8] IL-15Rα recycles and presents IL-15 in trans to neighboring cells
    Dubois, S
    Mariner, J
    Waldmann, TA
    Tagaya, Y
    [J]. IMMUNITY, 2002, 17 (05) : 537 - 547
  • [9] DUMONT DJ, 1992, ONCOGENE, V7, P1471
  • [10] DOMINANT-NEGATIVE AND TARGETED NULL MUTATIONS IN THE ENDOTHELIAL RECEPTOR TYROSINE KINASE, TEK, REVEAL A CRITICAL ROLE IN VASCULOGENESIS OF THE EMBRYO
    DUMONT, DJ
    GRADWOHL, G
    FONG, GH
    PURI, MC
    GERTSENSTEIN, M
    AUERBACH, A
    BREITMAN, ML
    [J]. GENES & DEVELOPMENT, 1994, 8 (16) : 1897 - 1909