A Role for Codon Order in Translation Dynamics

被引：274

作者：

Cannarrozzi, Gina ^{[1
,3
]}

Schraudolph, Nicol N. ^{[1
]}

Faty, Mahamadou ^{[2
]}

von Rohr, Peter ^{[1
]}

Friberg, Markus T. ^{[1
]}

Roth, Alexander C. ^{[1
,3
]}

Gonnet, Pedro ^{[1
]}

Gonnet, Gaston ^{[1
,3
]}

Barral, Yves ^{[2
]}

机构：

[1] ETH, Inst Computat Sci, CH-8092 Zurich, Switzerland

[2] ETH, Inst Biochem, CH-8093 Zurich, Switzerland

[3] Swiss Inst Bioinformat, CH-1015 Lausanne, Switzerland

来源：

CELL | 2010年 / 141卷 / 02期

基金：

瑞士国家科学基金会;

关键词：

AMINOACYL-TRANSFER-RNA; SACCHAROMYCES-CEREVISIAE; PROTEIN-SYNTHESIS; EXPRESSION PROFILES; GENE-EXPRESSION; CELL-CYCLE; USAGE; YEAST; BIAS; GENOME;

D O I：

10.1016/j.cell.2010.02.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genetic code is degenerate. Each amino acid is encoded by up to six synonymous codons; the choice between these codons influences gene expression. Here, we show that in coding sequences, once a particular codon has been used, subsequent occurrences of the same amino acid do not use codons randomly, but favor codons that use the same tRNA. The effect is pronounced in rapidly induced genes, involves both frequent and rare codons and diminishes only slowly as a function of the distance between subsequent synonymous codons. Furthermore, we found that in S. cerevisiae codon correlation accelerates translation relative to the translation of synonymous yet anticorrelated sequences. The data suggest that tRNA diffusion away from the ribosome is slower than translation, and that some tRNA channeling takes place at the ribosome. They also establish that the dynamics of translation leave a significant signature at the level of the genome.

引用

页码：355 / 367

页数：13

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