Regenerating corticospinal fibers in the Marmoset (Callitrix jacchus) after spinal cord lesion and treatment with the anti-Nogo-A antibody IN-1

被引:107
作者
Fouad, K [1 ]
Klusman, I
Schwab, ME
机构
[1] Univ Alberta, Fac Rehabil Med, Edmonton, AB T6G 2G4, Canada
[2] Univ Zurich, Brain Res Inst, Zurich, Switzerland
[3] Swiss Fed Inst Technol, Dept Biol, Zurich, Switzerland
关键词
myelin-associated inhibitors; primates; repair; spinal cord injury;
D O I
10.1111/j.1460-9568.2004.03716.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Neutralizing the myelin-associated growth inhibitor Nogo-A in adult spinal cord-injured rats can promote regeneration of injured and compensatory sprouting of uninjured axons. Nogo-A is present in humans, making its neutralization a possible novel treatment option for paraplegic patients. In this study we examined the effects of an extensively used anti-Nogo-A antibody (mAb IN-1) on the regenerative capabilities of lesioned corticospinal tract (CST) axons in a primate, the Marmoset monkey. Unilateral thoracic lesions of the CST were performed in six adult Marmosets, followed by the application of mAb IN-1 into the cerebrospinal fluid circulation by a graft of hybridoma cells. A unilateral injection of biotin dextran amine into the motor cortex was performed to analyse sprouting and regeneration of the lesioned axons. In the control antibody-treated animal CST fibers stopped rostral to the lesion site and often showed retraction bulbs. In contrast, in four out of five mAb IN-1-treated animals fine labeled neurites had grown into, through and around the lesion site. Thus, this study provides first anatomical evidence that in primates, the neutralization of the myelin-associated inhibitor Nogo-A results in increased regenerative sprouting and growth of lesioned spinal cord axons.
引用
收藏
页码:2479 / 2482
页数:4
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