Novel fluo-4 analogs for fluorescent calcium measurements

被引：22

作者：

Martin, VV

Beierlein, M

Morgan, JL

Rothe, A

Gee, KR

机构：

[1] Mol Probes Invitrogen Detect Technol, Eugene, OR 97492 USA

[2] Harvard Univ, Sch Med, Dept Neurobiol, Boston, MA 02115 USA

[3] Washington Univ, Sch Med, Dept Anat & Neurobiol, St Louis, MO 63110 USA

来源：

CELL CALCIUM | 2004年 / 36卷 / 06期

关键词：

dextran; protein; reactive; Ca2+; streptavidin; biotin; fluorescence; neuron;

D O I：

10.1016/j.ceca.2004.05.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown to be functional in brain slices. All new probes were characterized spectroscopically and exhibited large fluorescence increases upon calcium-binding. The biotinylated version of fluo-4 formed a persistent streptavidin complex which still responded to increasing calcium concentrations with a large fluorescence increase. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：509 / 514

页数：6

共 22 条

[1] Local calcium signaling in neurons [J].