Expression of various multidrug resistance-associated protein (MRP) homologues in brain microvessel endothelial cells

被引：166

作者：

Zhang, Y ^{[1
]}

Han, HY ^{[1
]}

Elmquist, WF ^{[1
]}

Miller, DW ^{[1
]}

机构：

[1] Univ Nebraska, Coll Pharm, Dept Pharmaceut Sci, Nebraska Med Ctr 986025, Omaha, NE 68198 USA

来源：

BRAIN RESEARCH | 2000年 / 876卷 / 1-2期

关键词：

blood-brain barrier; multidrug resistance-associated protein; brain microvessel; reverse transcriptase-polymerase chain reaction; Western blot;

D O I：

10.1016/S0006-8993(00)02628-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Multidrug resistance-associated protein (MRP) actively transports a broad range of anionic compounds out of the cell. To date, six different homologues of MRP (i.e. MRP1-MRP6) have been identified. The current study examines the expression of the various MRP homologues in both primary cultured bovine brain microvessel endothelial cells (BBMEC) and the capillary-enriched fraction from bovine brain homogenates. RT-PCR analysis demonstrated the presence of MRP1, MRP4, MRP5 and MRP6 in both BBMEC and the capillary-enriched fractions of brain homogenates. While low levels of MRP3 were detected in the BBMEC, it was not observed in the capillary-enriched fraction. In addition, RT-PCR and Western blot studies indicated an absence of MRP3 expression in both blued-brain barrier preparations. The presence of several different MRP homologues in the brain microvessel endothelial cells may be important in controlling the permeability of the blood-brain barrier to organic anions. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：148 / 153

页数：6

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