Characterization of a novel fucose-regulated promoter (PfcsK) suitable for gene essentiality and antibacterial mode-of-action studies in Streptococcus pneumoniae

被引：76

作者：

Chan, PF ^{[1
]}

O'Dwyer, KM ^{[1
]}

Palmer, LM ^{[1
]}

Ambrad, JD ^{[1
]}

Ingraham, KA ^{[1
]}

So, C ^{[1
]}

Lonetto, MA ^{[1
]}

Biswas, S ^{[1
]}

Rosenberg, M ^{[1
]}

Holmes, DJ ^{[1
]}

Zalacain, M ^{[1
]}

机构：

[1] GlaxoSmithKline Pharmaceut, Microbial Musculoskeletal & Proliferat Dis CEDD, Collegeville, PA 19426 USA

来源：

JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 06期

关键词：

D O I：

10.1128/JB.185.6.2051-2058.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The promoter of the Streptococcus pneumoniae putative fuculose kinase gene (fcsK), the first gene of a novel fucose utilization operon, is induced by fucose and repressed by glucose or sucrose. When the streptococcal polypeptide deformylase (PDF) gene (def1, encoding PDF) was placed under the control of P-fcsK, fucose-dependent growth of the S. pneumoniae (P-fcsK::defl) strain was observed, confirming the essential nature of PDF in this organism. The mode of antibacterial action of actinonin, a known PDF inhibitor, was also confirmed with this strain. The endogenous fuculose kinase promoter is a tightly regulated, titratable promoter which will be useful for target validation and for confirmation of the mode of action of novel antibacterial drugs in S. pneumoniae.

引用

页码：2051 / 2058

页数：8

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