Single-cell electroporation

被引:165
作者
Wang, Manyan [1 ]
Orwar, Owe [2 ]
Olofsson, Jessica [2 ]
Weber, Stephen G. [1 ]
机构
[1] Univ Pittsburgh, Dept Chem, Pittsburgh, PA 15260 USA
[2] Chalmers Univ Technol, Dept Phys Chem, S-41296 Gothenburg, Sweden
关键词
Cell membrane; Electric field; Microelectrodes; Microfluidics; Transfection; MICRO-ELECTROPORATION; MAMMALIAN-CELLS; PATCH-CLAMP; MODELING ELECTROPORATION; MEMBRANE PERTURBATION; ELLIPSOIDAL CELLS; SMALL MOLECULES; ELECTRIC-FIELD; GENE-TRANSFER; STEM-CELLS;
D O I
10.1007/s00216-010-3744-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Single-cell electroporation (SCEP) is a relatively new technique that has emerged in the last decade or so for single-cell studies. When a large enough electric field is applied to a single cell, transient nano-pores form in the cell membrane allowing molecules to be transported into and out of the cell. Unlike bulk electroporation, in which a homogenous electric field is applied to a suspension of cells, in SCEP an electric field is created locally near a single cell. Today, single-cell-level studies are at the frontier of biochemical research, and SCEP is a promising tool in such studies. In this review, we discuss pore formation based on theoretical and experimental approaches. Current SCEP techniques using microelectrodes, micropipettes, electrolyte-filled capillaries, and microfabricated devices are all thoroughly discussed for adherent and suspended cells. SCEP has been applied in in-vivo and in-vitro studies for delivery of cell-impermeant molecules such as drugs, DNA, and siRNA, and for morphological observations.
引用
收藏
页码:3235 / 3248
页数:14
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