The mRNA-like noncoding RNA Gomafu constitutes a novel nuclear domain in a subset of neurons

被引:245
作者
Sone, Masamitsu
Hayashi, Tetsutaro
Tarui, Hiroshi
Agata, Kiyokazu
Takeichi, Masatoshi
Nakagawa, Shinichi
机构
[1] RIKEN, Nakagawa Intiat Res Unit, Wako, Saitama 3510198, Japan
[2] RIKEN, Ctr Dev Biol, Chuo Ku, Kobe, Hyogo 6500047, Japan
[3] Kyoto Univ, Grad Sch Biostudies, Dept Cell & Dev Biol, Kyoto 6068502, Japan
关键词
mRNA export; Noncoding RNA; nuclear domains; nuclear matrix;
D O I
10.1242/jcs.009357
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Recent transcriptome analyses have revealed that a large body of noncoding regions of mammalian genomes are actually transcribed into RNAs. Our understanding of the molecular features of these noncoding RNAs is far from complete. We have identified a novel mRNA-like noncoding gene, named Gomafu, which is expressed in a distinct set of neurons in the mouse nervous system. Interestingly, spliced mature Gomafu RNA is localized to the nucleus despite its mRNA-like characteristics, which usually act as potent export signals to the cytoplasm. Within the nucleus, Gomafu RNA is detected as numerous spots that do not colocalize with known nuclear domain markers. Gomafu RNA is extremely insoluble and remains intact after nuclear matrix preparation. Furthermore, heterokaryon assays revealed that Gomafu RNA does not shuttle between the nucleus and cytoplasm, but is retained in the nucleus after its transcription. We propose that Gomafu RNA represents a novel family of mRNA-like noncoding RNA that constitutes a cell-type-specific component of the nuclear matrix.
引用
收藏
页码:2498 / 2506
页数:9
相关论文
共 50 条
[1]   The functions of animal microRNAs [J].
Ambros, V .
NATURE, 2004, 431 (7006) :350-355
[2]  
Andersen JS, 2002, CURR BIOL, V12, P1, DOI 10.1016/S0960-9822(01)00650-9
[3]   THE NUCLEAR MATRIX - A HEURISTIC MODEL FOR INVESTIGATING GENOMIC ORGANIZATION AND FUNCTION IN THE CELL-NUCLEUS [J].
BEREZNEY, R .
JOURNAL OF CELLULAR BIOCHEMISTRY, 1991, 47 (02) :109-123
[4]   Genomic analysis of mouse retinal development [J].
Blackshaw, S ;
Harpavat, S ;
Trimarchi, J ;
Cai, L ;
Huang, HY ;
Kuo, WP ;
Weber, G ;
Lee, K ;
Fraioli, RE ;
Cho, SH ;
Yung, R ;
Asch, E ;
Ohno-Machado, L ;
Wong, WH ;
Cepko, CL .
PLOS BIOLOGY, 2004, 2 (09) :1411-1431
[5]  
BRADY G, 1993, METHOD ENZYMOL, V225, P611
[6]   The Air noncoding RNA:: An imprinted cis-silencing transcript [J].
Braidotti, G ;
Baubec, T ;
Pauler, F ;
Seidl, C ;
Smrzka, O ;
Stricker, S ;
Yotova, I ;
Barlow, DP .
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 2004, 69 :55-66
[7]   X-chromosome inactivation:: closing in on proteins that bind XistRNA [J].
Brockdorff, N .
TRENDS IN GENETICS, 2002, 18 (07) :352-358
[8]  
Cao XW, 2006, ANNU REV NEUROSCI, V29, P77, DOI 10.1146/annurev.neuro.29.051605.112839
[9]   The transcriptional landscape of the mammalian genome [J].
Carninci, P ;
Kasukawa, T ;
Katayama, S ;
Gough, J ;
Frith, MC ;
Maeda, N ;
Oyama, R ;
Ravasi, T ;
Lenhard, B ;
Wells, C ;
Kodzius, R ;
Shimokawa, K ;
Bajic, VB ;
Brenner, SE ;
Batalov, S ;
Forrest, ARR ;
Zavolan, M ;
Davis, MJ ;
Wilming, LG ;
Aidinis, V ;
Allen, JE ;
Ambesi-Impiombato, X ;
Apweiler, R ;
Aturaliya, RN ;
Bailey, TL ;
Bansal, M ;
Baxter, L ;
Beisel, KW ;
Bersano, T ;
Bono, H ;
Chalk, AM ;
Chiu, KP ;
Choudhary, V ;
Christoffels, A ;
Clutterbuck, DR ;
Crowe, ML ;
Dalla, E ;
Dalrymple, BP ;
de Bono, B ;
Della Gatta, G ;
di Bernardo, D ;
Down, T ;
Engstrom, P ;
Fagiolini, M ;
Faulkner, G ;
Fletcher, CF ;
Fukushima, T ;
Furuno, M ;
Futaki, S ;
Gariboldi, M .
SCIENCE, 2005, 309 (5740) :1559-1563
[10]   DISCRETE NUCLEAR DOMAINS OF POLY(A) RNA AND THEIR RELATIONSHIP TO THE FUNCTIONAL-ORGANIZATION OF THE NUCLEUS [J].
CARTER, KC ;
TANEJA, KL ;
LAWRENCE, JB .
JOURNAL OF CELL BIOLOGY, 1991, 115 (05) :1191-1202