Activation of restriction enzyme by electrochemically released magnesium ion

被引：8

作者：

Katsura, S ^{[1
]}

Harada, N ^{[1
]}

Maeda, Y ^{[1
]}

Komatsu, J ^{[1
]}

Matsuura, S ^{[1
]}

Takashima, K ^{[1
]}

Mizuno, A ^{[1
]}

机构：

[1] Toyohashi Univ Technol, Dept Ecol Engn, Toyohashi, Aichi 4418580, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2004年 / 98卷 / 04期

关键词：

micromanipulation; micro-operation; micro electro mechanical system (MEMS) microreactor;

D O I：

10.1016/S1389-1723(04)00284-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Observation and cutting of DNA molecules at intended positions permit several new experimental methods that are completely different from conventional molecular biology methods; therefore several cutting methods have been proposed and studied. In this paper. a new cutting method for a DNA molecule by localizing the activity of a restriction enzyme is presented. Since most restriction enzymes require magnesium ions for their activation, local restriction enzyme activity can be controlled by the local concentration of magnesium ions. Applying a direct current (dc) voltage to a needle electrode of metallic magnesium made it possible to control the local magnesium ion concentration at the tip of the needle. The restriction enzyme was activated only, when magnesium ions were electrochemically supplied.

引用

页码：293 / 297

页数：5

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