Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (Glossina morsitans morsitans)

被引：22

作者：

Attardo, Geoffrey M. ^{[1
]}

Ribeiro, Jose M. C. ^{[2
]}

Wu, Yineng ^{[1
]}

Berriman, Matthew ^{[3
]}

Aksoy, Serap ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Epidemiol & Publ Hlth, New Haven, CT 06510 USA

[2] NIAID, Sect Vector Biol, Lab Malaria & Vector Res, NIH, Rockville, MD USA

[3] Wellcome Trust Sanger Inst, Hinxton CB10 1SA, England

来源：

BMC GENOMICS | 2010年 / 11卷

基金：

英国惠康基金;

关键词：

MOLECULAR ASPECTS; GENE ONTOLOGY; YOLK PROTEINS; PSI-BLAST; EXPRESSION; DATABASE; PHYLOGENY; BINDING; CLONING;

D O I：

10.1186/1471-2164-11-160

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Background: Tsetse flies, vectors of African trypanosomes, undergo viviparous reproduction (the deposition of live offspring). This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female's lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse (Glossina morsitans morsitans) reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed. Results: Analysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries (salivary gland, fat body/milk gland, and midgut) identified 51 that are unique to the reproductive/immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects (hypothetical conserved). The analysis also yielded seven putative proteins without significant homology to sequences present in the public database (unknown genes). These proteins may represent unique functions associated with tsetse's viviparous reproductive cycle. RTPCR analysis of hypothetical conserved and unknown contigs was performed to determine basic tissue and stage specificity of the expression of these genes. Conclusion: This paper identifies 51 putative proteins specific to a tsetse reproductive/ immature EST library. 11 of these proteins correspond to hypothetical conserved genes and 7 proteins are tsetse specific.

引用

页数：10

共 42 条

[1]

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2]

An insight into the sialome of Glossina morsitans morsitans [J].

Alves-Silva, Juliana ;

Ribeiro, Jose M. C. ;

Van Den Abbeele, Jan ;

Attardo, Geoffrey ;

Hao, Zhengrong ;

Haines, Lee R. ;

Soares, Marcelo B. ;

Berriman, Matthew ;

Aksoy, Serap ;

Lehane, Michael J. .

BMC GENOMICS, 2010, 11

[3]

Gene Ontology: tool for the unification of biology [J].

Ashburner, M ;

Ball, CA ;

Blake, JA ;

Botstein, D ;

Butler, H ;

Cherry, JM ;

Davis, AP ;

Dolinski, K ;

Dwight, SS ;

Eppig, JT ;

Harris, MA ;

Hill, DP ;

Issel-Tarver, L ;

Kasarskis, A ;

Lewis, S ;

Matese, JC ;

Richardson, JE ;

Ringwald, M ;

Rubin, GM ;

Sherlock, G .

NATURE GENETICS, 2000, 25 (01) :25-29

[4]

Analysis of fat body transcriptome from the adult tsetse fly, Glossina morsitans morsitans [J].

Attardo, G. M. ;

Strickler-Dinglasan, P. ;

Perkin, S. A. H. ;

Caler, E. ;

Bonaldo, M. F. ;

Soares, M. B. ;

El-Sayeed, N. ;

Aksoy, S. .

INSECT MOLECULAR BIOLOGY, 2006, 15 (04) :411-424

[5]

Molecular aspects of viviparous reproductive biology of the tsetse fly (Glossina morsitans morsitans):: Regulation of yolk and milk gland protein synthesis [J].

Attardo, Geoffrey M. ;

Guz, Nurper ;

Strickler-Dinglasan, Patricia ;

Aksoy, Serap .

JOURNAL OF INSECT PHYSIOLOGY, 2006, 52 (11-12) :1128-1136

[6]

Bateman A, 2004, NUCLEIC ACIDS RES, V32, pD138, DOI [10.1093/nar/gkp985, 10.1093/nar/gkh121, 10.1093/nar/gkr1065]

[7]

3 GENES FOR 3 YOLK PROTEINS IN DROSOPHILA-MELANOGASTER [J].

BOWNES, M .

FEBS LETTERS, 1979, 100 (01) :95-98

[8]

DYNAMICS OF PREGNANCY CYCLE IN TSETSE GLOSSINA-MORSITANS [J].

DENLINGER, DL ;

MA, WC .

JOURNAL OF INSECT PHYSIOLOGY, 1974, 20 (06) :1015-1026

[9]

Evolution and phylogeny of the diptera: A molecular phylogenetic analysis using 28S rDNA sequences [J].

Friedrich, M ;

Tautz, D .

SYSTEMATIC BIOLOGY, 1997, 46 (04) :674-698

[10]

Gleeson JG, 1999, ANN NEUROL, V45, P146, DOI 10.1002/1531-8249(199902)45:2<146::AID-ANA3>3.0.CO

← 1 2 3 4 5 →