Evaluation of Platelet-Derived Extracellular Vesicles in Gingival Fibroblasts and Keratinocytes for Periodontal Applications

被引：15

作者：

Antich-Rossello, Miquel ^{[1
,2
]}

Munar-Bestard, Marta ^{[1
,2
]}

Forteza-Genestra, Maria Antonia ^{[1
,2
]}

Calvo, Javier ^{[1
,2
,3
]}

Gaya, Antoni ^{[1
,2
,3
]}

Monjo, Marta ^{[1
,2
,4
]}

Ramis, Joana M. ^{[1
,2
,4
]}

机构：

[1] Univ Balearic Isl UIB, Res Inst Hlth Sci Iun, Cell Therapy & Tissue Engn Grp, Ctra Valldemossa Km 7-5, Palma De Mallorca 07122, Spain

[2] Hlth Res Inst Balearic Isl IdISBa, Carretera Valldemossa 79, Palma De Mallorca 07120, Spain

[3] Fundacio Banc Sang & Teixits Illes Balears FBSTIB, Carrer Rossello & Cazador 20, Palma De Mallorca 07004, Spain

[4] Univ Balearic Isl UIB, Dept Biol Fonamental & Ciencies La Salut, Ctra Valldemossa Km 7-5, Palma De Mallorca 07122, Spain

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 14期

关键词：

extracellular vesicles; platelet lysate; in vitro wound healing; hyaluronic acid; regeneration; gingival fibroblasts; gingival keratinocytes; RICH PLASMA; CELL BIOLOGY; PROLIFERATION; MIGRATION; EXOSOMES; PROMOTE;

D O I：

10.3390/ijms23147668

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Gingival regeneration aims at restoring the architecture and functionality of oral damaged tissue. Different biomaterials or biological materials have been tested for tissue repair, such as platelet concentrates such as PL. In this article, the use of extracellular vesicles (EVs) derived from platelet lysate (PL) and their combination with hyaluronic acid biomaterials (HA) in an in vitro wound healing assay is investigated. EVs were isolated by size exclusion chromatography from PL. In addition, HA gels were formulated with PL or EVs. EVs or HA combined with EVs (HA-EVs) were tested in vitro in gingival fibroblasts and keratinocytes for biocompatibility (LDH activity and metabolic activity) and by an in vitro wound-healing assay and gene expression analysis. EVs and EVs-HA treatments were biocompatible in gingival fibroblasts and keratinocytes and showed an increase in wound healing in vitro compared to control. Moreover, changes in gene expression related to extracellular matrix remodeling were observed after the treatment with EVs. EVs can be combined with HA biomaterials, showing good biocompatibility and preserving their activity and functionality. Therefore, platelet-derived EVs could emerge as a new application for periodontal regeneration in combination with biomaterials in order to enhance their clinical use.

引用

页数：16

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