Visualizing "green oil" in live algal cells

被引：168

作者：

Cooper, Mark Scott ^{[1
]}

Hardin, William Robert ^{[1
]}

Petersen, Timothy Wayne ^{[2
]}

Cattolico, Rose Ann ^{[1
]}

机构：

[1] Univ Washington, Dept Biol, Seattle, WA 98195 USA

[2] BD Biosci, Adv Cytometry Grp, Seattle, WA 98125 USA

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2010年 / 109卷 / 02期

关键词：

BODIPY; 505/515; Vital stain; Lipid body; Oleaginous; FACS; Algae; LIPID-CONTENT; FLUORESCENCE;

D O I：

10.1016/j.jbiosc.2009.08.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report here that BODIPY 505/515, a green lipophilic fluorescent dye, serves as an excellent vital stain for the oil-containing lipid bodies of live algal cells. BODIPY 505/515 vital staining can be used in combination with fluorescent activated cell sorting to detect and isolate algal cells possessing high lipid content. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

引用

收藏

页码：198 / 201

页数：4

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