Determination at ppb level of an anti-human immunodeficiency virus nucleoside drug by capillary electrophoresis-electrospray ionization tandem mass spectrometry

被引:42
作者
Cahours, X [1 ]
Dessans, H [1 ]
Morin, P [1 ]
Dreux, M [1 ]
Agrofoglio, L [1 ]
机构
[1] Univ Orleans, CNRS, UPRESA 6005, Inst Chim Organ & Analyt, F-45067 Orleans, France
关键词
pharmaceutical analysis; capillary electrophoresis; mass spectrometry; nucleosides; didanosine; didexoyadenosine-5 '-triphosphate;
D O I
10.1016/S0021-9673(00)00524-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Capillary electrophoresis coupled with tandem mass spectrometry was used to indirectly separate and quantify the active metabolite of the anti-human immunodeficiency virus (anti-HIV) didanosine drug. The influence of several parameters (pH and ionic strength of volatile formic acid-ammonia buffer) upon electroosmotic flow, electrophoretic mobility and peak efficiency of several nucleosides (A, dA, ddA, C) has been studied. This paper illustrates the current importance in CE-MS technique as a complementary or substituted method to the known HPLC-radioimmunoassay or HPLC-UV method to measure levels of anti-HIV drugs. The limit of detection for 2',3'-dideoxyadenosine by this method is 2 mug l(-1) in a formic acid-ammonia buffer (pH 2,5, 10 mM ionic strength). This methodology could be used to perform simultaneous detection of two or more anti-HIV nucleosides, such as stavudine or didanosine in combination therapy. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:101 / 109
页数:9
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