DNA electrotransfer:: its principles and an updated review of its therapeutic applications

被引：190

作者：

André, F ^{[1
]}

Mir, LM ^{[1
]}

机构：

[1] Inst Gustave Roussy, CNRS, UMR 8121, Lab Vectorol & Gene Transfer, F-94805 Villejuif, France

来源：

GENE THERAPY | 2004年 / 11卷 / Suppl 1期

关键词：

electrogenetherapy; DNA electrotransfer; nonviral gene therapy; electroporation; electropermeabilization; naked DNA;

D O I：

10.1038/sj.gt.3302367

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of electric pulses to transfect all types of cells is well known and regularly used in vitro for bacteria and eukaryotic cells transformation. Electric pulses can also be delivered in vivo either transcutaneously or with electrodes in direct contact with the tissues. After injection of naked DNA in a tissue, appropriate local electric pulses can result in a very high expression of the transferred genes. This manuscript describes the evolution in the concepts and the various optimization steps that have led to the use of combinations of pulses that fit with the known roles of the electric pulses in DNA electrotransfer, namely cell electropermeabilization and DNA electrophoresis. A summary of the main applications published until now is also reported, restricted to the in vivo preclinical trials using therapeutic genes.

引用

页码：S33 / S42

页数：10

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