Mitochondria isolated in nearly isotonic KCl buffer: Focus on cardiolipin and organelle morphology

被引：31

作者：

Corcelli, Angela ^{[1
,2
]}

Saponetti, Matilde Sublimi ^{[1
]}

Zaccagnino, Patrizia ^{[1
]}

Lopalco, Patrizia ^{[1
]}

Mastrodonato, Maria ^{[4
]}

Liquori, Giuseppa E. ^{[4
]}

Lorusso, Michele ^{[1
,3
]}

机构：

[1] Univ Bari, Dept Med Biochem Biol & Phys, I-70125 Bari, Italy

[2] CNR, IPCF, I-70126 Bari, Italy

[3] CNR, Inst Biomembrane & Bioenerget, I-70126 Bari, Italy

[4] CNR, Dept Anim & Environm Biol, I-70126 Bari, Italy

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2010年 / 1798卷 / 03期

关键词：

Mitochondrion; KCl based isolation buffer; Mitochondrial lipids; Cardiolipin; Cristae morphology; LINKED MECHANICAL ACTIVITY; CYTOCHROME-C; RHODOBACTER-SPHAEROIDES; ULTRASTRUCTURAL BASES; ELECTRON-TRANSPORT; OSMOTIC-STRESS; MEMBRANE; LIVER; BIOSYNTHESIS; CURVATURE;

D O I：

10.1016/j.bbamem.2010.01.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat liver mitochondria were isolated in parallel in two different isolation buffers: a standard buffer containing mannitol/sucrose and a nearly physiological KCl based solution. The two different organelle preparations were comparatively characterized by respiratory activity, heme content, microsomal and Golgi contamination, electron microscopy and lipid analyses. The substitution of saccharides with KCl in the isolation buffer does not induce the formation of mitoplasts or disruption of mitochondria. Mitochondria isolated in KCl buffer are coupled and able to maintain a stable transmembrane charge separation. A number of biochemical and functional differences between the two organelle preparations are described; in particular KCl mitochondria exhibit lower cardiolipin content and smaller intracristal compartments in comparison with the standard mitochondrial preparation. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：681 / 687

页数：7

共 25 条

[1] 58K, a microtubule-binding golgi protein, is a formiminotransferase cyclodeaminase [J].