Arachidonic acid causes cytochrome c release from heart mitochondria

被引：31

作者：

Di Paola, M

Cocco, T

Lorusso, M

机构：

[1] Univ Bari, Policlin, Dept Med Biochem & Biol, I-70124 Bari, Italy

[2] CNR, Ctr Study Mitochondria & Energy Metab, I-70126 Bari, Italy

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 277卷 / 01期

关键词：

arachidonic acid; cytochrome c release; heart mitochondria; mitochondrial swelling; permeability transition; cell death;

D O I：

10.1006/bbrc.2000.3653

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Arachidonic acid interaction with heart mitochondria is known to cause uncoupling as well as inhibition of pyruvate + malate and succinate-supported respiration. Here we present experiments showing that arachidonic acid causes cytochrome c release from Ca2+-loaded heart mitochondria. We have also measured mitochondrial matrix swelling and found a fairly good correlation between the two processes, as revealed by the same arachidonic acid concentration dependence and by the same susceptibility toward different free fatty acid species. The effects produced by arachidonic acid are not related to its protonophoric activity since, under the experimental conditions used, saturating concentrations of FCCP did not cause any effect. (C) 2000 Academic Press.

引用

收藏

页码：128 / 133

页数：6

相关论文

共 37 条

[1] Cytochrome c release from brain mitochondria is independent of the mitochondrial permeability transition [J].

Andreyev, AY ;

Fahy, B ;

Fiskum, G .

FEBS LETTERS, 1998, 439 (03) :373-376

[2] Rapid spectrophotometric method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited [J].

Appaix, F ;

Minatchy, MN ;

Riva-Lavieille, C ;

Olivares, J ;

Antonsson, B ;

Saks, VA .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2000, 1457 (03) :175-181

[3] Mitochondria and cell death - Mechanistic aspects and methodological issues [J].

Bernardi, P ;

Scorrano, L ;

Colonna, R ;

Petronilli, V ;

Di Lisa, F .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 264 (03) :687-701

[4]

BERNARDI P, 1992, J BIOL CHEM, V267, P8834

[5] Inhibition of the mitochondrial permeability transition by cyclosporin a during long time frame experiments: Relationship between pore opening and the activity of mitochondrial phospholipases [J].

Broekemeier, KM ;

Pfeiffer, DR .

BIOCHEMISTRY, 1995, 34 (50) :16440-16449

[6] INTERACTION OF CYTOCHROME-C WITH CYTOCHROME-BC1 COMPLEX OF THE MITOCHONDRIAL RESPIRATORY-CHAIN [J].

BROGER, C ;

NALECZ, MJ ;

AZZI, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 592 (03) :519-527

[7] CYCLOSPORINE-A SUPPRESSION OF UNCOUPLING IN LIVER-MITOCHONDRIA OF GROUND-SQUIRREL DURING AROUSAL FROM HIBERNATION [J].

BRUSTOVETSKY, NN ;

EGOROVA, MV ;

GNUTOV, DY ;

MOKHOVA, EN ;

SKULACHEV, VP .

FEBS LETTERS, 1993, 315 (03) :233-236

[8] Mitochondrial control of apoptosis:: the role of cytochrome c [J].

Cai, JY ;

Yang, J ;

Jones, DP .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1998, 1366 (1-2) :139-149

[9] The participation of pyridine nucleotides redox state and reactive oxygen in the fatty acid-induced permeability transition in rat liver mitochondria [J].

Catisti, R ;

Vercesi, AE .

FEBS LETTERS, 1999, 464 (1-2) :97-101

[10] Carboxyatractyloside increases the effect of oleate on mitochondrial permeability transition [J].

Chávez, E ;

Zazueta, C ;

García, N .

FEBS LETTERS, 1999, 445 (01) :189-191

← 1 2 3 4 →