High-level expression of functional recombinant human coagulation factor VII in insect cells

被引：16

作者：

Masroori, Nasser ^{[1
]}

Halabian, Raheleh ^{[1
]}

Mohammadipour, Mahshid ^{[1
]}

Roushandeh, Amaneh Mohammadi ^{[2
]}

Rouhbakhsh, Mahdi ^{[1
]}

Najafabadi, Ali Jahanian ^{[3
]}

Fathabad, Mahdi Edalati ^{[1
]}

Salimi, Mohamad ^{[4
]}

Shokrgozar, Mohamad Ali ^{[5
]}

Roudkenar, Mehryar Habibi ^{[1
]}

机构：

[1] Iranian Blood Transfus Org, Res Ctr, Iranian Blood Transfus Org, Tehran, Iran

[2] Med Univ Tabriz, Dept Anat, Fac Med, Tabriz, Iran

[3] Pasteur Inst Iran, Dept Mol Biol, Tehran, Iran

[4] Iran Univ Med Sci, Dept Biotechnol, Tehran, Iran

[5] Pasteur Inst Iran, Natl Cell Bank Iran, Tehran, Iran

来源：

BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 06期

关键词：

Baculovirus; gamma-carboxylase; Coagulation FVII; Factor VII; Insect cell; GAMMA-GLUTAMYL CARBOXYLASE; HEMOPHILIA; PURIFICATION; INHIBITORS; EFFICACY; MUTATION; PROTEIN;

D O I：

10.1007/s10529-010-0227-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinant coagulation factor VII (FVII) is used as a potential therapeutic intervention in hemophilia patients who produce antibodies against the coagulation factors. Mammalian cell lines provide low levels of expression, however, the Spodoptera frugiperda Sf9 cell line and baculovirus expression system are powerful systems for high-level expression of recombinant proteins, but due to the lack of endogenous vitamin K-dependent carboxylase, expression of functional FVII using this system is impossible. In the present study, we report a simple but versatile method to overcome the defect for high-level expression of the functional recombinant coagulation FVII in Sf9 cells. This method involves simultaneous expression of both human gamma-carboxylase (hGC) and human FVII genes in the host. It may be possible to express other vitamin K-dependent coagulation factors using this method in the future.

引用

页码：803 / 809

页数：7

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