Rapid identification of new bacterial alcohol dehydrogenases for (R)- and (S)-enantioselective reduction of β-ketoesters

被引：21

作者：

Zhang, J ^{[1
]}

Duetz, WA ^{[1
]}

Witholt, B ^{[1
]}

Li, Z ^{[1
]}

机构：

[1] ETH, Inst Biotechnol, CH-8093 Zurich, Switzerland

来源：

CHEMICAL COMMUNICATIONS | 2004年 / 18期

关键词：

D O I：

10.1039/b407004f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

New bacterial alcohol dehydrogenases with high and complementary enantioselectivity for the reduction of ethyl 3-keto-4,4,4-trifluorobutyrate 1 and methyl 3-keto-3-(3'-pyridyl)- propionate 3 have been rapidly identified by use of a new methodology consisting of preselection of microorganisms based on degradation ability and high-throughput screening with a miniaturized system coupled with fast analysis of enantioselectivities.

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页码：2120 / 2121

页数：2

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