Hydrogen sulphide regulates intracellular pH in vascular smooth muscle cells

被引：95

作者：

Lee, Shiau Wei ^{[1
]}

Cheng, Yvonne ^{[1
]}

Moore, Philip K. ^{[1
]}

Bian, Jin-Song ^{[1
]}

机构：

[1] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Pharmacol, Cardiovasc Biol Res Grp, Singapore 117597, Singapore

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 358卷 / 04期

基金：

英国医学研究理事会;

关键词：

hydrogen sulphide; smooth muscle cell; intracellular pH; vascular contractility;

D O I：

10.1016/j.bbrc.2007.05.063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the role of hydrogen sulphide (H2S) in intracellular pH (pH(i)) regulation in vascular smooth muscle cells and its contribution on vasodilation. NaHS, a H2S donor, decreased pH(i) in a concentration-dependent manner ranging from 10 mu M to 1 mM. Neither inhibition of the Na+/H+ exchanger with 5-(N-ethyl-N-isopropyl) amiloride, (EIPA, 10 mu M), nor plasmalemnial Ca2+-ATPase with CdCl2 (20 nM) alters the effect of NaHS on pH(i). Blockade of the Cl-/HCO3- exchanger with 4,4 '-diisothiocyanatostilbene-2,2 '-disulfonic acid (DIDS) significantly attenuated the pHi lowering effect of NaHS. Moreover, NaHS significantly increased the activity of Cl-/HCO3- exchanger when measured with NH4Cl Prepulse method. DIDS attenuated the vasorelaxation induced by NaHS whereas EIPA and CdCl2 did not cause any change. In conclusion, H2S induced intracellular acidification via activation of Cl-/HCO3- exchanger, which is, at least partially, responsible for H2S-mediated vasorelaxation. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：1142 / 1147

页数：6

共 24 条

[1] Abe K, 1996, J NEUROSCI, V16, P1066
[2] INTERACTION OF CD-2+ WITH THE CALMODULIN-ACTIVATED (CA-2+ + MG-2+)-ATPASE ACTIVITY OF HUMAN-ERYTHROCYTE GHOSTS
AKERMAN, KEO
HONKANIEMI, J
SCOTT, IG
ANDERSSON, LC
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 845 (01) : 48 - 53
[3] Regulation of vascular nitric oxide in vitro and in vivo; a new role for endogenous hydrogen sulphide?
Ali, M. Y.
Ping, C. Y.
Mok, Y-Y P.
Ling, L.
Whiteman, M.
Bhatia, M.
Moore, P. K.
[J]. BRITISH JOURNAL OF PHARMACOLOGY, 2006, 149 (06) : 625 - 634
[4] AN INVESTIGATION OF INTRINSIC BUFFERING POWER IN RAT VASCULAR SMOOTH-MUSCLE CELLS
AUSTIN, C
WRAY, S
[J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1995, 429 (03): : 325 - 331
[5] Role of hydrogen sulfide in the cardioprotection caused by ischemic preconditioning in the rat heart and cardiac myocytes
Bian, JS
Yong, QC
Pan, TT
Feng, ZN
Ali, MY
Zhou, SF
Moore, PK
[J]. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2006, 316 (02) : 670 - 678
[6] Hyperactivity and altered mRNA isoform expression of the Cl-/HCO-3 anion-exchanger in the hypertrophied myocardium
de Cingolani, GC
Morgan, P
Mundiña-Weilenmann, C
Casey, J
Fujinaga, J
de Hurtado, MC
Cingolani, H
[J]. CARDIOVASCULAR RESEARCH, 2001, 51 (01) : 71 - 79
[7] Angiotensin II activates Na+-independent Cl--HCO3- exchange in ventricular myocardium
de Hurtado, MCC
Alvarez, BV
Pérez, NG
Ennis, IL
Cingolani, HE
[J]. CIRCULATION RESEARCH, 1998, 82 (04) : 473 - 481
[8] Vertebrate phylogeny of hydrogen sulfide vasoactivity
Dombkowski, RA
Russell, MJ
Schulman, AA
Doellman, MM
Olson, KR
[J]. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2005, 288 (01) : R243 - R252
[9] Hydrogen sulfide as an endogenous regulator of vascular smooth muscle tone in trout
Dombkowski, RA
Russell, MJ
Olson, KR
[J]. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2004, 286 (04) : R678 - R685
[10] EFFECT OF NH4CL ON THE CONTRACTILITY OF ISOLATED VASCULAR SMOOTH-MUSCLE
FURTADO, MR
[J]. LIFE SCIENCES, 1987, 41 (01) : 95 - 102

← 1 2 3 →