Hydrogen sulphide regulates intracellular pH in vascular smooth muscle cells

被引：95

作者：

Lee, Shiau Wei ^{[1
]}

Cheng, Yvonne ^{[1
]}

Moore, Philip K. ^{[1
]}

Bian, Jin-Song ^{[1
]}

机构：

[1] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Pharmacol, Cardiovasc Biol Res Grp, Singapore 117597, Singapore

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 358卷 / 04期

基金：

英国医学研究理事会;

关键词：

hydrogen sulphide; smooth muscle cell; intracellular pH; vascular contractility;

D O I：

10.1016/j.bbrc.2007.05.063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the role of hydrogen sulphide (H2S) in intracellular pH (pH(i)) regulation in vascular smooth muscle cells and its contribution on vasodilation. NaHS, a H2S donor, decreased pH(i) in a concentration-dependent manner ranging from 10 mu M to 1 mM. Neither inhibition of the Na+/H+ exchanger with 5-(N-ethyl-N-isopropyl) amiloride, (EIPA, 10 mu M), nor plasmalemnial Ca2+-ATPase with CdCl2 (20 nM) alters the effect of NaHS on pH(i). Blockade of the Cl-/HCO3- exchanger with 4,4 '-diisothiocyanatostilbene-2,2 '-disulfonic acid (DIDS) significantly attenuated the pHi lowering effect of NaHS. Moreover, NaHS significantly increased the activity of Cl-/HCO3- exchanger when measured with NH4Cl Prepulse method. DIDS attenuated the vasorelaxation induced by NaHS whereas EIPA and CdCl2 did not cause any change. In conclusion, H2S induced intracellular acidification via activation of Cl-/HCO3- exchanger, which is, at least partially, responsible for H2S-mediated vasorelaxation. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：1142 / 1147

页数：6

共 24 条

[21] Mechanisms of action of pH-induced effects on vascular smooth muscle
Wray, S
Smith, RD
[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2004, 263 (01) : 163 - 172
[22] NA-INDEPENDENT CL--HCO3- EXCHANGE MEDIATES RECOVERY OF PH(I) FROM ALKALOSIS IN GUINEA-PIG VENTRICULAR MYOCYTES
XU, P
SPITZER, KW
[J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 267 (01): : H85 - H91
[23] MOLECULAR-CLONING, EXPRESSION, AND CHROMOSOMAL LOCALIZATION OF 2 ISOFORMS OF THE AE3 ANION-EXCHANGER FROM HUMAN HEART
YANNOUKAKOS, D
STUARTTILLEY, A
FERNANDEZ, HA
FEY, P
DUYK, G
ALPER, SL
[J]. CIRCULATION RESEARCH, 1994, 75 (04) : 603 - 614
[24] The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener
Zhao, WM
Zhang, J
Lu, YJ
Wang, R
[J]. EMBO JOURNAL, 2001, 20 (21) : 6008 - 6016

← 1 2 3 →