High-performance liquid chromatography coupled to nuclear magnetic resonance spectroscopy -: Application to the ecdysteroids of Silene otites

被引：22

作者：

Wilson, ID ^{[1
]}

Morgan, ED

Lafont, R

Wright, B

机构：

[1] Univ Keele, Dept Chem, Keele ST5 5BG, Staffs, England

[2] Ecole Normale Super, Dept Biol, CNRS EP 119, F-75230 Paris 05, France

[3] Zeneca Pharmaceut, Macclesfield SK10 4TG, Cheshire, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 799卷 / 1-2期

关键词：

nuclear magnetic resonance spectroscopy; Silene otitis; ecdysteroids;

D O I：

10.1016/S0021-9673(97)01299-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-performance liquid chromatography (HPLC) coupled to nuclear magnetic resonance (NMR) spectroscopy has been used to obtain H-1 NMR spectra of ecdysteroids present in an extract of the plant Silene otites. Reversed-phase gradient chromatography was performed using a (H2O)-H-2-acetonitrile-trifluoroacetic acid-based solvent system. Diagnostic NMR spectra were obtained for 20-hydroxyecdysone, 2-deoxy-20-hydroxyecdysone and 2-deoxyecdysone using the stopped-flow HPLC-NMR technique. The compounds were present in the extracts in amounts between ca. 12 and 135 mu g on-column (as determined using HPLC-UV). (C) 1998 Elsevier Science B.V.

引用

页码：333 / 336

页数：4

共 9 条

[1] ONLINE USE OF NMR DETECTION IN SEPARATION CHEMISTRY [J].