Endogenous amylin contributes to the anorectic effects of cholecystokinin and bombesin

被引：40

作者：

Mollet, A ^{[1
]}

Meier, S ^{[1
]}

Grabler, V ^{[1
]}

Gilg, S ^{[1
]}

Scharrer, E ^{[1
]}

Lutz, TA ^{[1
]}

机构：

[1] Univ Zurich, Inst Vet Physiol, CH-8057 Zurich, Switzerland

来源：

PEPTIDES | 2003年 / 24卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

amylin; CCK; BBS; food intake; interaction; plasma amylin level; amylin-deficient mice;

D O I：

10.1016/S0196-9781(02)00280-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies indicated that amylin contributes to the anorectic effects of cholecystokinin (CCK) and bombesin (BBS), possibly by enhancing the release of pancreatic amylin or by modulating their anorectic actions within the central nervous system (CNS). To elucidate the interaction between amylin and CCK or BBS, respectively, we investigated the influence of an IP injection of CCK or BBS on feeding in amylin-deficient mice (IAPP(-/-)). The anorectic effects of CCK and BBS were nearly abolished in IAPP-/- mice compared to wildtype (WT) mice (e.g. 20 mug/kg CCK, 1-h food intake: WT/NaCl 0.53 +/- 0.03 g; WT/CCK 0.16 +/- 0.03 g (P < 0.001); IAPP(-/-)/NaCl 0.49 +/- 0.05 g; IAPP(-/-)/CCK 0.39 +/- 0.04 g). Acute amylin replacement restored the anorectic effect of CCK in IAPP-/mice. To find out whether CCK or BBS enhance the feeding-induced release of pancreatic amylin, we injected rats with CCK-8 (0.5-50 mu g/kg) or BBS (5 mu g/kg) and measured plasma amylin levels after injections. Neither CCK nor BBS increased the plasma amylin level in rats. We suggest that the mediation of the anorectic effects of CCK and BBS by amylin is not dependent on a CCK- or BBS-induced release of pancreatic amylin, but may rather be due to a modulation of their effects by amylin within the CNS. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：91 / 98

页数：8

共 33 条

[1] ISOLATION AND STRUCTURE OF BOMBESIN AND ALYTESIN, 2 ANALOGOUS ACTIVE PEPTIDES FROM SKIN OF EUROPEAN AMPHIBIANS BOMBINA AND ALYTES [J].

ANASTASI, A ;

ERSPAMER, V ;

BUCCI, M .

EXPERIENTIA, 1971, 27 (02) :166-&

[2] Behaviorally specific inhibition of sham feeding by amylin [J].

Asarian, L ;

Eckel, LA ;

Geary, N .

PEPTIDES, 1998, 19 (10) :1711-1718

[3] Synergy between amylin and cholecystokinin for inhibition of food intake in mice [J].

Bhavsar, S ;

Watkins, J ;

Young, A .

PHYSIOLOGY & BEHAVIOR, 1998, 64 (04) :557-561

[4] INTRAPERITONEAL INJECTIONS OF NANOGRAM CCK-8 DOSES INHIBIT FEEDING IN RATS [J].

CANOVA, A ;

GEARY, N .

APPETITE, 1991, 17 (03) :221-227

[5] Multiple amylin receptors arise from receptor activity-modifying protein interaction with the calcitonin receptor gene product [J].

Christopoulos, G ;

Perry, KJ ;

Morfis, M ;

Tilakaratne, N ;

Gao, YY ;

Fraser, NJ ;

Main, MJ ;

Foord, SM ;

Sexton, PM .

MOLECULAR PHARMACOLOGY, 1999, 56 (01) :235-242

[6] AMYLIN COMPARED WITH CALCITONIN-GENE-RELATED PEPTIDE - STRUCTURE, BIOLOGY, AND RELEVANCE TO METABOLIC DISEASE [J].

COOPER, GJS .

ENDOCRINE REVIEWS, 1994, 15 (02) :163-201

[7] Increased insulin secretion and glucose tolerance in mice lacking islet amyloid polypeptide (amylin) [J].

Gebre-Medhin, S ;

Mulder, H ;

Pekny, M ;

Westermark, G ;

Törnell, J ;

Westermark, P ;

Sundler, F ;

Ahrén, B ;

Betsholtz, C .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 250 (02) :271-277

[8] CHOLECYSTOKININ DECREASES FOOD INTAKE IN RATS [J].

GIBBS, J ;

YOUNG, RC ;

SMITH, GP .

JOURNAL OF COMPARATIVE AND PHYSIOLOGICAL PSYCHOLOGY, 1973, 84 (03) :488-495

[9] BOMBESIN STIMULATES THE RELEASE OF INSULIN AND GLUCAGON, BUT NOT PANCREATIC SOMATOSTATIN, FROM THE ISOLATED PERFUSED DOG PANCREAS [J].

IPP, E ;

UNGER, RH .

ENDOCRINE RESEARCH COMMUNICATIONS, 1979, 6 (01) :37-42

[10]

Liddle RA., 1994, Gut peptides: biochemistry and physiology, P175

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