A higher-plant type ζ-carotene desaturase in the cyanobacterium Synechocystis PCC6803

被引:48
作者
Breitenbach, J
Fernández-González, B
Vioque, A
Sandmann, G
机构
[1] Univ Frankfurt, Inst Bot, Biosynth Grp, D-60054 Frankfurt, Germany
[2] Univ Sevilla, CSIC, Inst Bioquim Vegetal & Fotosintesis, Seville 41092, Spain
关键词
zeta-carotene desaturase gene; J852; lycopene; neurosporene; phylogeny; Synechocystis PCC6803;
D O I
10.1023/A:1005997405431
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The genomic DNA sequence of Synechocystis was analysed for putative zeta-carotene desaturase genes. Two promising candidates slr0940 and slr0033 were found with similarities to the structurally different zeta-carotene desaturase genes from higher plants and Anabaena, respectively. Only the expression product of the analogue to the plant gene, slr0940, was able to mediate the 2-step desaturation of zeta-carotene via neurosporene to lycopene after complementation of this pathway in Escherichia coli. When enzyme reactions were carried out with this protein, activity was obtained with either zeta-carotene or neuroporene as substrates. The in vitro reaction was inhibited by the pyrimidine derivative J852 which is effective as experimental herbicide in plants. The occurrence of two different types of zeta-carotene desaturases among cyanobacteria and the phylogenetic consequences on chloroplast evolution are discussed.
引用
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页码:725 / 732
页数:8
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