Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4

被引：100

作者：

Ito, M ^{[1
]}

Takasaki, T ^{[1
]}

Yamada, KI ^{[1
]}

Nerome, R ^{[1
]}

Tajima, S ^{[1
]}

Kurane, I ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Virol 1, Shinjyuku Ku, Tokyo 1628640, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 12期

关键词：

D O I：

10.1128/JCM.42.12.5935-5937.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively.

引用

页码：5935 / 5937

页数：3

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