Fine physical mapping of the rice stripe resistance gene locus, Stvb-i

被引：43

作者：

Hayano-Saito, Y

Saito, K

Nakamura, S

Kawasaki, S

Iwasaki, M

机构：

[1] Hokkaido Natl Agr Expt Stn, Toyohira Ku, Sapporo, Hokkaido 0628555, Japan

[2] Natl Inst Agrobiol Resources, Tsuchiura, Ibaraki 3058602, Japan

[3] Tohoku Natl Agr Expt Stn, Morioka, Iwate 0200123, Japan

来源：

THEORETICAL AND APPLIED GENETICS | 2000年 / 101卷 / 1-2期

关键词：

rice; stripe disease resistance gene; Stvb-i; BAC; physical map;

D O I：

10.1007/s001220051449

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The Stvb-i gene confers stripe disease resistance to rice. For positional cloning, we constructed a physical map spanning 1.8-cM distance between flanking markers, consisting of 18 bacterial artificial chromosome (BAC) clones, around the Stvb-i locus on rice chromosome 11. The 18 clones were isolated by screening a BAC library derived from a japonica cultivar, Shimokita, with three Stvb-i-linked RFLP markers and DraI-digested DNAs of a yeast artificial chromosome (YAC) clone. The results of Southern hybridization and restriction enzyme analyses indicated that these BAC clones are contiguous and cover about a 700-kb region containing the Stvb-i allele. Utilizing end and internal fragments of the BAC insert DNAs, 33 molecular markers were generated within a small chromosomal region including the Stvb-i locus. Genotyping analysis with these markers for a resistant cultivar and four nearby recombinants selected from 120 F-2 individuals indicated that Stvb-i is contained within an approximately 286-kb region covered with two overlapping BAC clones.

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页码：59 / 63

页数：5

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