Engineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability

被引：57

作者：

Liu, Wenjin ^{[1
]}

Zhang, Xiao-Zhou ^{[1
]}

Zhang, Zuoming ^{[1
,2
]}

Zhang, Y. -H. Percival ^{[1
,3
,4
]}

机构：

[1] Virginia Polytech Inst & State Univ, Dept Biol Syst Engn, Blacksburg, VA 24061 USA

[2] Jilin Univ, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130023, Peoples R China

[3] Virginia Polytech Inst & State Univ, ICTAS, Blacksburg, VA 24061 USA

[4] BESC, Dept Energy, Oak Ridge, TN 37831 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 14期

关键词：

CELLULOSE-BINDING DOMAINS; TRICHODERMA-REESEI; PROCESSIVE ENDOGLUCANASE; CRYSTALLINE CELLULOSE; ENZYMATIC-HYDROLYSIS; SURFACE DISPLAY; LIGNOCELLULOSE; CELLULOVORANS; ADSORPTION; EVOLUTION;

D O I：

10.1128/AEM.00958-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

A family 5 glycoside hydrolase from Clostridium phytofermentans was cloned and engineered through a cellulase cell surface display system in Escherichia coli. The presence of cell surface anchoring, a cellulose binding module, or a His tag greatly influenced the activities of wild-type and mutant enzymes on soluble and solid cellulosic substrates, suggesting the high complexity of cellulase engineering. The best mutant had 92%, 36%, and 46% longer half-lives at 60 degrees C on carboxymethyl cellulose, regenerated amorphous cellulose, and Avicel, respectively.

引用

页码：4914 / 4917

页数：4

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