Detection of nucleotide polymorphisms in feline calicivirus isolates by reverse transcription PCR and a fluorescence resonance energy transfer probe

被引：9

作者：

Helps, C ^{[1
]}

Harbour, D ^{[1
]}

机构：

[1] Univ Bristol, Dept Vet Clin Sci, Div Vet Pathol Infect & Immun, Bristol BS40 5DU, Avon, England

来源：

JOURNAL OF VIROLOGICAL METHODS | 2003年 / 109卷 / 02期

关键词：

FCV; nucleotide polymorphisms; FRET;

D O I：

10.1016/S0166-0934(03)00051-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fluorescence resonance energy transfer system has been developed to detect nucleotide polymorphisms in isolates of feline calicivirus (FCV). Isolates with an exact match to the 28 nucleotide probe gave a melting temperature of 75degreesC while isolates with one, two, three or four mismatches gave melting temperatures of 71, 65, 60 and 57degreesC, respectively. The technique, which is simple, rapid and accurate, is suitable for typing field isolates of FCV. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：261 / 263

页数：3

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