Claspin, a novel protein required for the activation of Chk1 during a DNA replication checkpoint response in Xenopus egg extracts

被引：231

作者：

Kumagai, A ^{[1
]}

Dunphy, WG ^{[1
]}

机构：

[1] CALTECH, Howard Hughes Med Inst, Div Biol, Pasadena, CA 91125 USA

来源：

MOLECULAR CELL | 2000年 / 6卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S1097-2765(05)00092-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified Claspin, a novel protein that binds to Xenopus Chk1 (Xchk1). Binding of Claspin to Xchk1 is highly elevated in the presence of DNA templates that trigger a checkpoint arrest of the cell cycle in Xenopus egg extracts. Xchk1 becomes phosphorylated during a checkpoint response, and we demonstrate directly that this phosphorylation results in the activation of Xchk1. Immunodepletion of Claspin from egg extracts abolishes both the phosphorylation and activation of Xchk1. Furthermore, Claspin-depleted extracts are unable to arrest the cell cycle in response to DNA replication blocks. Taken together, these findings indicate that Claspin is an essential upstream regulator of Xchk1.

引用

页码：839 / 849

页数：11

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