Selective inhibition of Alu retrotransposition by APOBEC3G

被引：116

作者：

Hulme, Amy E. ^{[1
]}

Bogerd, Hal P.

Cullen, Bryan R.

Moran, John V.

机构：

[1] Univ Michigan, Sch Med, Dept Human Genet, Ann Arbor, MI 48109 USA

[2] Duke Univ, Med Ctr, Ctr Virol, Durham, NC 27710 USA

[3] Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA

[4] Univ Michigan, Sch Med, Dept Internal Med, Ann Arbor, MI 48109 USA

来源：

GENE | 2007年 / 390卷 / 1-2期

关键词：

LINE-1; retrotransposon; APOBEC; transposable element;

D O I：

10.1016/j.gene.2006.08.032

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The non-LTR retrotransposon LINE-1 (L1) comprises similar to 17% of the human genome, and the L1-encoded proteins can function in trans to mediate the retrotransposition of non-autonomous retrotransposons (i.e., Alu and probably SVA elements) and cellular mRNAs to generate processed pseudogenes. Here, we have examined the effect of APOBEC3G and APOBEC3F, cytidine deaminases that inhibit Vif-deficient HIV-1 replication, on Alu retrotransposition and other L1-mediated retrotransposition processes. We demonstrate that APOBEC3G selectively inhibits Alu retrotransposition in an OR-F I p-in dependent manner. An active cytidine deaminase site is not required for the inhibition of Alu retrotransposition and the resultant integration events lack G to A or C to T hypermutation. These data demonstrate a differential restriction of L1 and Alu retrotransposition by APOBEC3G, and suggest that the Alu ribonucleoprotein complex may be targeted by APOBEC3G. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：199 / 205

页数：7

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