Non-viral and hybrid vectors in human gene therapy: an update

被引：172

作者：

Schmidt-Wolf, GD ^{[1
]}

Schmidt-Wolf, IGH ^{[1
]}

机构：

[1] Univ Bonn, Med Klin & Poliklin 1, D-5300 Bonn, Germany

来源：

TRENDS IN MOLECULAR MEDICINE | 2003年 / 9卷 / 02期

关键词：

D O I：

10.1016/S1471-4914(03)00005-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Non-viral DNA vectors have several advantages over viral vectors. For example, virus production is expensive and there are safety concerns regarding viral manipulations. In addition, the size of the delivered plasmid is limited by the size of the viral capsid, whereas this is not a problem with non-viral vectors. The major disadvantage of using non-viral DNA delivery vectors, compared with their viral counterparts, is the low transfection efficiency. This has resulted in low levels of usage in clinical trials. Consequently, the majority of research into non-viral gene therapy has been focused on developing more efficient vectors.

引用

页码：67 / 72

页数：6

共 50 条

[11] Immunotherapy of advanced malignancy by direct gene transfer of an interleukin-2 DNA/DMRIE/DOPE lipid complex: Phase I/II experience [J].

Galanis, E ;

Hersh, EM ;

Stopeck, AT ;

Gonzalez, R ;

Burch, P ;

Spier, C ;

Akporiaye, ET ;

Rinehart, JJ ;

Edmonson, J ;

Sobol, RE ;

Forscher, C ;

Sondak, VK ;

Lewis, BD ;

Unger, EC ;

O'Driscoll, M ;

Selk, L ;

Rubin, J .

JOURNAL OF CLINICAL ONCOLOGY, 1999, 17 (10) :3313-3323

[12]

GUNZBURG WH, 1997, GENTRANSFER SAUGETIE, P19

[13] Targeting of the photocytotoxic compound AlPcS4 to HeLa cells by transferrin conjugated PEG-liposomes [J].

Gusens, A ;

Derycke, A ;

Missiaen, L ;

De Vos, D ;

Huwyler, J ;

Eberle, A ;

de Witte, P .

INTERNATIONAL JOURNAL OF CANCER, 2002, 101 (01) :78-85

[14] Development of biomaterials for gene therapy [J].

Han, S ;

Mahato, RI ;

Sung, YK ;

Kim, SW .

MOLECULAR THERAPY, 2000, 2 (04) :302-317

[15] An improved plasmid DNA expression vector for direct injection into skeletal muscle [J].

Hartikka, J ;

Sawdey, M ;

CornefertJensen, F ;

Margalith, M ;

Barnhart, K ;

Nolasco, M ;

Vahlsing, HL ;

Meek, J ;

Marquet, M ;

Hobart, P ;

Norman, J ;

Manthorpe, M .

HUMAN GENE THERAPY, 1996, 7 (10) :1205-1217

[16] Folate-targeted gene transfer in vivo [J].

Hofland, HEJ ;

Masson, C ;

Iginla, S ;

Osetinsky, I ;

Reddy, JA ;

Leamon, CP ;

Scherman, D ;

Bessodes, M ;

Wils, P .

MOLECULAR THERAPY, 2002, 5 (06) :739-744

[17] Tumor regression by targeted gene delivery to the neovasculature [J].

Hood, JD ;

Bednarski, M ;

Frausto, R ;

Guccione, S ;

Reisfeld, RA ;

Xiang, R ;

Cheresh, DA .

SCIENCE, 2002, 296 (5577) :2404-2407

[18] CANCER GENE-THERAPY USING PLASMID DNA - PURIFICATION OF DNA FOR HUMAN CLINICAL-TRIALS [J].

HORN, NA ;

MEEK, JA ;

BUDAHAZI, G ;

MARQUET, M .

HUMAN GENE THERAPY, 1995, 6 (05) :565-573

[19]

HUANG CH, 1999, NONVIRAL VECTORS GEN

[20] INCREASED EXPRESSION OF DNA COINTRODUCED WITH NUCLEAR-PROTEIN IN ADULT-RAT LIVER [J].

KANEDA, Y ;

IWAI, K ;

UCHIDA, T .

SCIENCE, 1989, 243 (4889) :375-378

← 1 2 3 4 5 →