Topical antibody delivery systems produce sustained levels in mucosal tissue and blood

被引:24
作者
Kuo, PY
Sherwood, JK
Saltzman, WM [1 ]
机构
[1] Cornell Univ, Sch Chem Engn, Ithaca, NY 14853 USA
[2] Johns Hopkins Univ, Dept Chem Engn, Baltimore, MD 21218 USA
关键词
drug delivery; controlled-release; passive immunization;
D O I
10.1038/nbt0298-163
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Immunity at mucosal surfaces, which are ports of entry for many pathogens, is essential in preventing infections. But most current strategies for passive immunization involve injection of antibodies for systemic, not mucosal, protection. We measured mucosal and systemic antibody levels after controlled topical delivery to the vagina. Poly(ethylene-co-vinyl acetate) disks containing I-125-labeled monoclonal IgG or anti-lactate dehydrogenase-C, antibodies were placed in the vaginas of mice. High antibody levels (0.26-12 mu g/ml) were maintained at the mucosal surface for 7 days after disk insertion. Antibody molecules also penetrated into the vaginal epithelium, presumably by diffusing through the extracellular space, and entered the circulation. Biologically active antibodies were detected in the blood. The antibody concentration in the blood was approximately 1% of the concentration in the vagina. Although the permeability of the epithelium to macromolecules is low, high concentrations were maintained at the luminal surface for an extended period, permitting substantial systemic uptake of antibody.
引用
收藏
页码:163 / 167
页数:5
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