Capture of DNA in microfluidic channel using magnetic beads: Increasing capture efficiency with integrated microfluidic mixer

被引：40

作者：

Lund-Olesen, T. ^{[1
]}

Dufva, M. ^{[1
]}

Hansen, M. F. ^{[1
]}

机构：

[1] Tech Univ Denmark, MIC Dept Micro & Nanotechnol, DK-2800 Lyngby, Denmark

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2007年 / 311卷 / 01期

关键词：

magnetic bead; magnetic separation; microfluidic; bioseparation; lab on a chip;

D O I：

10.1016/j.jmmm.2006.10.1171

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We have studied the hybridization of target DNA in solution with probe DNA on magnetic beads immobilized on the channel sidewalls in a magnetic bead separator. The hybridization is carried out under a liquid flow and is diffusion limited. Two systems are compared: one with a straight microfluidic channel and one with an integrated staggered herringbone mixer. Fluorescence microscopy studies show that the hybridization is much more efficient in the system with the integrated mixer. The results, which are discussed in terms of a simple model, are relevant for any diffusion-limited reaction taking place on the surface in a microfluidic system. (C) 2006 Elsevier B.V. All rights reserved.

引用

页码：396 / 400

页数：5

共 14 条

[1] Gains in sensitivity with a device that mixes microarray hybridization solution in a 25-μm-thick chamber
Adey, NB
Lei, M
Howard, MT
Jensen, JD
Mayo, DA
Butel, DL
Coffin, SC
Moyer, TC
Slade, DE
Spute, MK
Hancock, AM
Eisenhoffer, GT
Dalley, BK
McNeely, MR
[J]. ANALYTICAL CHEMISTRY, 2002, 74 (24) : 6413 - 6417
[2] Hybridization enhancement using microfluidic planetary centrifugal mixing
Bynum, MA
Gordon, GB
[J]. ANALYTICAL CHEMISTRY, 2004, 76 (23) : 7039 - 7044
[3] Characterisation of Dynabeads® by magnetization measurements and Mossbauer spectroscopy
Fonnum, G
Johansson, C
Molteberg, A
Morup, S
Aksnes, E
[J]. JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2005, 293 (01) : 41 - 47
[4] HANSEN MF, 2006, P INT 2006 SAN DIEG, P247
[5] Hybridization enhancement using cavitation microstreaming
Liu, RH
Lenigk, R
Druyor-Sanchez, RL
Yang, JN
Grodzinski, P
[J]. ANALYTICAL CHEMISTRY, 2003, 75 (08) : 1911 - 1917
[6] Lund-Olesen T, 2006, PROC IEEE MICR ELECT, P386
[7] Quantitative characterization of magnetic separators: Comparison of systems with and without integrated microfluidic mixers
Lund-Olesen, Torsten
Bruus, Henrik
Hansen, Mikkel Fougt
[J]. BIOMEDICAL MICRODEVICES, 2007, 9 (02) : 195 - 205
[8] Polymer microfluidic chip for online monitoring of microarray hybridizations
Noerholm, M
Bruus, H
Jakobsen, MH
Telleman, P
Ramsing, NB
[J]. LAB ON A CHIP, 2004, 4 (01) : 28 - 37
[9] Enhancement of DNA micro-array analysis using a shear-driven micro-channel flow system
Pappaert, K
Vanderhoeven, J
Van Hummelen, P
Dutta, B
Clicq, D
Baron, GV
Desmet, G
[J]. JOURNAL OF CHROMATOGRAPHY A, 2003, 1014 (1-2) : 1 - 9
[10] Active mixing during hybridization improves the accuracy and reproducibility of microarray results
Schaupp, CJ
Jiang, GJ
Myers, TG
Wilson, MA
[J]. BIOTECHNIQUES, 2005, 38 (01) : 117 - 119

← 1 2 →