Dynamic Expression of a LEF-EGFP WNT Reporter in Mouse Development and Cancer

被引：27

作者：

Currier, Nicolas ^{[1
]}

Chea, Kathleen ^{[1
]}

Hlavacova, Mirka ^{[1
]}

Sussman, Daniel J. ^{[2
]}

Seldin, David C. ^{[1
]}

Dominguez, Isabel ^{[1
]}

机构：

[1] Boston Univ, Sch Med, Dept Med, Boston, MA 02118 USA

[2] New Horizons Diagnost Corp, Columbia, MD USA

来源：

GENESIS | 2010年 / 48卷 / 03期

关键词：

Wnt reporter; EGFP; embryo; beta-catenin; transgenic mouse; breast cancer; PROTEIN-KINASE CK2; CATENIN SIGNALING PATHWAY; BETA-CATENIN; AXIS FORMATION; MAMMARY-GLAND; EPITHELIAL-CELLS; XENOPUS EMBRYOS; MULTIPLE ROLES; TRANSCRIPTION; GENE;

D O I：

10.1002/dvg.20604

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

We have characterized a transgenic mouse line in which enhanced green fluorescent protein (EGFP) is expressed under the control of multimerized LEF-1 responsive elements. In embryos, EGFP was detected in known sites of Writ activation, including the primitive streak, mesoderm, neural tube, somites, heart, limb buds, mammary placodes, and whisker follicles. In vitro cultured transgenic embryonic fibroblasts upregulated EGFP expression in response to activation of Writ signaling by GSK3 beta inhibition. Mammary tumor cell lines derived from female LEF-EGFP transgenic mice treated with the carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) also express EGFR Thus, this transgenic line is useful for ex vivo and in vitro studies of Writ signaling in development and cancer. genesis 48:183-194, 2010. (C) 2010 Wiley-Liss, Inc.

引用

页码：183 / 194

页数：12

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