Section-illumination photoacoustic microscopy for dynamic 3D imaging of microcirculation in vivo

被引：34

作者：

Song, Liang ^{[1
]}

Maslov, Konstantin ^{[1
]}

Wang, Lihong V. ^{[1
]}

机构：

[1] Washington Univ, Dept Biomed Engn, Opt Imaging Lab, St Louis, MO 63130 USA

来源：

OPTICS LETTERS | 2010年 / 35卷 / 09期

关键词：

D O I：

10.1364/OL.35.001482

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We developed section-illumination photoacoustic microscopy capable of dynamic in vivo imaging of microvessels as small as 30 mu m in diameter. The section illumination improved the elevational resolution while an ultrasound array provided the in-plane axial and lateral resolutions. Using the imaging system, we monitored the wash-in dynamics of Evans Blue in the microcirculation of mouse ears at 249 Hz 2D and 0.5 Hz 3D image acquisition rates. Such observation allowed us to differentiate the arterioles from the venules. In the future, the technology may be used to study angiogenesis, diabetes-induced vascular complications, and pharmacokinetics. (C) 2010 Optical Society of America

引用

页码：1482 / 1484

页数：3

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